Document Detail

Aspartate-specific peptidases in Salmonella typhimurium: mutants deficient in peptidase E.
MedLine Citation:
PMID:  6086568     Owner:  NLM     Status:  MEDLINE    
The only dipeptide found to serve as a leucine source for a Salmonella strain lacking peptidases N, A, B, D, P, and Q was alpha-L-aspartyl-L-leucine. A peptidase (peptidase E) that specifically hydrolyzes Asp-X peptides was identified and partially purified from cell extracts. The enzyme (molecular weight, 35,000) is inactive toward dipeptides with N-terminal asparagine or glutamic acid. Mutants (pepE) lacking this enzyme were isolated by screening extracts for loss of the activity. Genetic mapping placed the pepE locus at 91.5 map units and established the gene order metA pepE zja-861::Tn5 malB. Duplications of the pepE locus showed a gene dosage effect on levels of peptidase E, suggesting that pepE is the structural gene for this enzyme. Mutations in pepE resulted in the loss of the ability to grow on Asp-Pro as a proline source but did not affect utilization of other dipeptides with N-terminal aspartic acid. Loss of peptidase E did not cause a detectable impairment in protein degradation. Two other peptidases present in cell extracts of mutants lacking peptidases N, A, B, D, P, Q, and E also hydrolyze many Asp-X dipeptides.
T H Carter; C G Miller
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  159     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1984 Aug 
Date Detail:
Created Date:  1984-09-17     Completed Date:  1984-09-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  453-9     Citation Subset:  IM    
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MeSH Terms
DNA Transposable Elements
Dipeptidases / genetics*,  isolation & purification,  metabolism
Methylnitronitrosoguanidine / toxicity
Salmonella typhimurium / drug effects,  enzymology*,  genetics
Species Specificity
Substrate Specificity
Grant Support
Reg. No./Substance:
0/DNA Transposable Elements; 70-25-7/Methylnitronitrosoguanidine; EC 3.4.13.-/Dipeptidases; EC 3.4.13.-/aspartyl peptidase E

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