| Aspartate dehydrogenase in vitamin B12-producing Klebsiella pneumoniae IFO 13541. | |
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MedLine Citation:
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PMID: 9819709 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We found a new reaction of aspartic acid dehydrogenation, catalyzed by NADP(+)-dependent aspartate dehydrogenase, in vitamin B12-producing Klebsiella pneumoniae IFO 13541. The enzyme, which was purified from a crude extract of K.pneumoniae IFO 13541, catalyzes the oxidative deamination of aspartic acid to form oxaloacetic acid. This enzyme had a molecular mass of about 124 kDa consisting of two identical subunits. L-Aspartic acid was a substrate, although D-aspartic acid and L-glutamic acid were inactive. The enzyme showed maximal activity at about pH 7.0-8.0 for the oxidative deamination of L-aspartic acid, and it required NADP+ as a coenzyme, while NAD+ was inactive. |
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Authors:
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T Okamura; H Noda; S Fukuda; M Ohsugi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of nutritional science and vitaminology Volume: 44 ISSN: 0301-4800 ISO Abbreviation: J. Nutr. Sci. Vitaminol. Publication Date: 1998 Aug |
Date Detail:
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Created Date: 1999-02-02 Completed Date: 1999-02-02 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0402640 Medline TA: J Nutr Sci Vitaminol (Tokyo) Country: JAPAN |
Other Details:
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Languages: eng Pagination: 483-90 Citation Subset: IM |
Affiliation:
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Department of Food Science and Nutrition, School of Human Environmental Sciences, Mukogawa Women's University, Nishinomiya, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Oxidoreductases
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isolation & purification*,
metabolism Ammonia / analysis Ammonium Sulfate / chemistry Aspartic Acid / analysis, metabolism* Chromatography, Agarose Chromatography, DEAE-Cellulose Chromatography, High Pressure Liquid Electrophoresis, Polyacrylamide Gel Hot Temperature Hydrogen-Ion Concentration Klebsiella pneumoniae / enzymology, metabolism* Molecular Weight NADP / metabolism Oxaloacetic Acids / analysis Protamines / chemistry Vitamin B 12 / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Oxaloacetic Acids; 0/Protamines; 53-59-8/NADP; 56-84-8/Aspartic Acid; 68-19-9/Vitamin B 12; 7664-41-7/Ammonia; 7783-20-2/Ammonium Sulfate; EC 1.4.-/Amino Acid Oxidoreductases; EC 1.4.1.-/aspartate dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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