Document Detail

Aspartate aminotransferase activity and isoenzyme proportions in human liver tissues.
MedLine Citation:
PMID:  213206     Owner:  NLM     Status:  MEDLINE    
Aspartate aminotransferase (EC activity and the distribution of its isoenzymes in human liver were examined. Rabbit antiserum against porcin soluble (i.e., non-mitochondrial) enzyme cross-reacted with the soluble enzyme of human origin and was used in an immunoprecipitation assay to quantitate the soluble and mitochondrial isoenzymes. These were separated by rapid, semiquantitative electrophoresis on cellulose acetate and by three other quantitative techniques: isoelectric focusing and anion-and cation-exchange chromatography. The mitochrondrial enzyme averaged 81% of the total activity in normal adult human liver (n = 4). Its contribution was dramatically reduced in single specimens of human fetal liver (56% of total activity) and hepatoblastoma tissue (38%). Total enzyme activities (mumol min-1 per gram of tissue) were: adult, 150; fetal, 38; tumor, 6. Total enzyme concentrations (micromoles of enzyme per kilogram of tissue) found were: adult, 10.8; fetal, 2.7; tumor, 0.4. The concentrations and isoenzyme distribution in human liver are compared to those in various animal model systems. Other methods for quantitative estimation of the isoenzymes and their adaptability for use in estimating concentrations in serum are reviewed.
R Rej
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  24     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1978 Nov 
Date Detail:
Created Date:  1979-01-15     Completed Date:  1979-01-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1971-9     Citation Subset:  IM    
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MeSH Terms
Aspartate Aminotransferases / analysis*
Carcinoma, Hepatocellular / enzymology
Isoelectric Focusing
Isoenzymes / analysis*
Liver / embryology,  enzymology*
Liver Neoplasms / enzymology
Middle Aged
Reference Values
Reg. No./Substance:
0/Isoenzymes; EC Aminotransferases

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