| Asparanin A induces G(2)/M cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells. | |
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MedLine Citation:
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PMID: 19254688 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We recently established that asparanin A, a steroidal saponin extracted from Asparagus officinalis L., is an active cytotoxic component. The molecular mechanisms by which asparanin A exerts its cytotoxic activity are currently unknown. In this study, we show that asparanin A induces G(2)/M phase arrest and apoptosis in human hepatocellular carcinoma HepG2 cells. Following treatment of HepG2 cells with asparanin A, cell cycle-related proteins such as cyclin A, Cdk1 and Cdk4 were down-regulated, while p21(WAF1/Cip1) and p-Cdk1 (Thr14/Tyr15) were up-regulated. Additionally, we observed poly (ADP-ribose) polymerase (PARP) cleavage and activation of caspase-3, caspase-8 and caspase-9. The expression ratio of Bax/Bcl-2 was increased in the treated cells, where Bax was also up-regulated. We also found that the expression of p53, a modulator of p21(WAF1/Cip1) and Bax, was not affected in asparanin A-treated cells. Collectively, our findings demonstrate that asparanin A induces cell cycle arrest and triggers apoptosis via a p53-independent manner in HepG2 cells. These data indicate that asparanin A shows promise as a preventive and/or therapeutic agent against human hepatoma. |
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Authors:
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Wei Liu; Xue-Feng Huang; Qi Qi; Qin-Sheng Dai; Li Yang; Fei-Fei Nie; Na Lu; Dan-Dan Gong; Ling-Yi Kong; Qing-Long Guo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-28 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 381 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-03-30 Completed Date: 2009-04-28 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 700-5 Citation Subset: IM |
Affiliation:
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Department of Physiology, China Pharmaceutical University, Nanjing, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents, Phytogenic
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pharmacology*,
therapeutic use Apoptosis* Asparagus Plant / chemistry CDC2 Protein Kinase / metabolism Carcinoma, Hepatocellular / drug therapy, metabolism*, prevention & control Cell Cycle / drug effects* Cell Division / drug effects Cell Line, Tumor Cyclin-Dependent Kinase 2 / metabolism Cyclin-Dependent Kinase Inhibitor p21 / metabolism G2 Phase / drug effects Humans Liver Neoplasms / drug therapy, metabolism*, prevention & control Saponins / pharmacology*, therapeutic use |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents, Phytogenic; 0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Saponins; 0/asparanin A; EC 2.7.11.22/CDC2 Protein Kinase; EC 2.7.11.22/Cyclin-Dependent Kinase 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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