Document Detail


Asparanin A induces G(2)/M cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells.
MedLine Citation:
PMID:  19254688     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We recently established that asparanin A, a steroidal saponin extracted from Asparagus officinalis L., is an active cytotoxic component. The molecular mechanisms by which asparanin A exerts its cytotoxic activity are currently unknown. In this study, we show that asparanin A induces G(2)/M phase arrest and apoptosis in human hepatocellular carcinoma HepG2 cells. Following treatment of HepG2 cells with asparanin A, cell cycle-related proteins such as cyclin A, Cdk1 and Cdk4 were down-regulated, while p21(WAF1/Cip1) and p-Cdk1 (Thr14/Tyr15) were up-regulated. Additionally, we observed poly (ADP-ribose) polymerase (PARP) cleavage and activation of caspase-3, caspase-8 and caspase-9. The expression ratio of Bax/Bcl-2 was increased in the treated cells, where Bax was also up-regulated. We also found that the expression of p53, a modulator of p21(WAF1/Cip1) and Bax, was not affected in asparanin A-treated cells. Collectively, our findings demonstrate that asparanin A induces cell cycle arrest and triggers apoptosis via a p53-independent manner in HepG2 cells. These data indicate that asparanin A shows promise as a preventive and/or therapeutic agent against human hepatoma.
Authors:
Wei Liu; Xue-Feng Huang; Qi Qi; Qin-Sheng Dai; Li Yang; Fei-Fei Nie; Na Lu; Dan-Dan Gong; Ling-Yi Kong; Qing-Long Guo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-28
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  381     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-30     Completed Date:  2009-04-28     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  700-5     Citation Subset:  IM    
Affiliation:
Department of Physiology, China Pharmaceutical University, Nanjing, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents, Phytogenic / pharmacology*,  therapeutic use
Apoptosis*
Asparagus Plant / chemistry
CDC2 Protein Kinase / metabolism
Carcinoma, Hepatocellular / drug therapy,  metabolism*,  prevention & control
Cell Cycle / drug effects*
Cell Division / drug effects
Cell Line, Tumor
Cyclin-Dependent Kinase 2 / metabolism
Cyclin-Dependent Kinase Inhibitor p21 / metabolism
G2 Phase / drug effects
Humans
Liver Neoplasms / drug therapy,  metabolism*,  prevention & control
Saponins / pharmacology*,  therapeutic use
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Saponins; 0/asparanin A; EC 2.7.11.22/CDC2 Protein Kinase; EC 2.7.11.22/Cyclin-Dependent Kinase 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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