Document Detail

Asparaginyl-tRNA synthetase from Escherichia coli has significant sequence homologies with yeast aspartyl-tRNA synthetase.
MedLine Citation:
PMID:  2693216     Owner:  NLM     Status:  MEDLINE    
The Escherichia coli asnS gene codes for asparaginyl-tRNA synthetase (NRSEC). We have sequenced the asnS region, including 382 bp of the 5'-untranslated region, 1398 bp of the coding region and 280 bp of the 3'-untranslated region. The DNA-derived NRSEC amino acid (aa) sequence was confirmed by direct aa sequencing of the N-terminal parts of the native protein and of a 28-kDa internal fragment generated by trypsin digestion. The asnS gene product has been purified to homogeneity using three chromatographic steps. Sequence comparison of the deduced NRSEC sequence with all aminoacyl-tRNA synthetase sequences showed significant homologies with the yeast aspartyl-tRNA synthetase and weaker relationships with other aminoacyl-tRNA synthetases for aa with an XAX codon.
J Anselme; M Härtlein
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Gene     Volume:  84     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  1989 Dec 
Date Detail:
Created Date:  1990-03-13     Completed Date:  1990-03-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  481-5     Citation Subset:  IM    
European Molecular Biology Laboratory, Grenoble, France.
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MeSH Terms
Amino Acid Sequence
Amino Acids / analysis
Amino Acyl-tRNA Synthetases / genetics*
Aspartate-tRNA Ligase / genetics*
Bacillus / enzymology,  genetics
Base Sequence
DNA, Bacterial
DNA, Fungal
Escherichia coli / enzymology,  genetics*
Molecular Sequence Data
RNA, Transfer, Amino Acyl*
Sequence Homology, Nucleic Acid
Yeasts / enzymology,  genetics
Reg. No./Substance:
0/Amino Acids; 0/Codon; 0/DNA, Bacterial; 0/DNA, Fungal; 0/RNA, Transfer, Amino Acyl; EC 6.1.1.-/Amino Acyl-tRNA Synthetases; EC Ligase; EC synthetase

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