| Asiatic acid, a pentacyclic triterpene from Centella asiatica, is neuroprotective in a mouse model of focal cerebral ischemia. | |
| | |
MedLine Citation:
|
PMID: 19382233 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Asiatic acid, a triterpenoid derivative from Centella asiatica, has shown biological effects such as antioxidant, antiinflammatory, and protection against glutamate- or beta-amyloid-induced neurotoxicity. We investigated the neuroprotective effect of asiatic acid in a mouse model of permanent cerebral ischemia. Various doses of asiatic acid (30, 75, or 165 mg/kg) were administered orally at 1 hr pre- and 3, 10, and 20 hr postischemia, and infarct volume and behavioral deficits were evaluated at day 1 or 7 postischemia. IgG (blood-brain barrier integrity) and cytochrome c (apoptosis) immunostaining was carried out at 24 hr postischemia. The effect of asiatic acid on stress-induced cytochrome c release was examined in isolated mitochondrial fractions. Furthermore, its effects on cell viability and mitochondrial membrane potential were studied in HT-22 cells exposed to oxygen-glucose deprivation. Asiatic acid significantly reduced the infarct volume by 60% at day 1 and by 26% at day 7 postischemia and improved neurological outcome at 24 hr postischemia. Our studies also showed that the neuroprotective properties of asiatic acid might be mediated in part through decreased blood-brain barrier permeability and reduction in mitochondrial injury. The present study suggests that asiatic acid may be useful in the treatment of cerebral ischemia. |
| | |
Authors:
|
Rajanikant G Krishnamurthy; Marie-Claude Senut; Daniel Zemke; Jiangyong Min; Mark B Frenkel; Eric J Greenberg; Seong-Woon Yu; Nick Ahn; John Goudreau; Mounzer Kassab; Kiran S Panickar; Arshad Majid |
Related Documents
:
|
11234913 - Determination of folate derivatives in rat tissues during folate deficiency. 6878453 - The maternal pheromone of the rat: testing some assumptions underlying a hypothesis. 11350053 - Overexpression of ucp-3 in skeletal muscle of mice results in increased expression of m... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of neuroscience research Volume: 87 ISSN: 1097-4547 ISO Abbreviation: J. Neurosci. Res. Publication Date: 2009 Aug |
Date Detail:
|
Created Date: 2009-07-07 Completed Date: 2009-09-21 Revised Date: 2011-01-12 |
Medline Journal Info:
|
Nlm Unique ID: 7600111 Medline TA: J Neurosci Res Country: United States |
Other Details:
|
Languages: eng Pagination: 2541-50 Citation Subset: IM |
Affiliation:
|
Division of Cerebrovascular Diseases and Department of Neurology and Ophthalmology, Michigan State University, East Lansing, Michigan 48824, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Brain / drug effects, metabolism, pathology Brain Ischemia / drug therapy* Cell Hypoxia Cell Line Cell Survival / drug effects Cytochromes c / metabolism Disease Models, Animal Glucose / deficiency Immunoglobulin G / metabolism Infarction, Middle Cerebral Artery / drug therapy* Male Membrane Potential, Mitochondrial / drug effects Mice Mice, Inbred C57BL Mitochondria / drug effects, metabolism Neuroprotective Agents / administration & dosage, therapeutic use* Pentacyclic Triterpenes Severity of Illness Index Time Factors Treatment Outcome Triterpenes / administration & dosage, therapeutic use* |
| Grant Support | |
ID/Acronym/Agency:
|
P30 AG028717-02/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Immunoglobulin G; 0/Neuroprotective Agents; 0/Pentacyclic Triterpenes; 0/Triterpenes; 464-92-6/asiatic acid; 50-99-7/Glucose; 9007-43-6/Cytochromes c |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Comparative anatomy and evolution of the cardiac innervation in New World monkeys (Platyrrhini, e. G...
Next Document: Functional and immunocytochemical characterization of D-serine transporters in cortical neuron and a...