| Asialoganglioside enhances the efficiency of gene transfection mediated by cationic liposomes with a cationic cholesterol derivative. | |
| | |
MedLine Citation:
|
PMID: 12951476 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We investigated the transfection efficiency mediated by asialoganglioside-containing cationic liposomes. Previously we reported that monosialoganglioside GM(1) (GM(1a)) enhanced transfection efficiency. In this study, we investigated the effects of sialic acid in gangliosides on transfection efficiency. Two mammalian culture cell lines HeLa and HepG2 were transfected with luciferase plasmids (pGL3) using cationic liposomes which contain monosialoganglioside GM(1) (GM(1a)) or its asialic counterpart, asialoganglioside GM(1) (GA(1)). Both GM(1a) and GA(1) enhanced the efficiency of transfection mediated by cationic liposomes, and GA(1) exhibited higher efficiency than GM(1a) in both cell lines. Transfection efficiency of ganglioside-containing liposomes was also assessed by the effects of antisense oligonucleotides (AS-ODN) for bcl-2 gene, which suppresses apoptotic cell death. Western blotting analysis revealed that the expression of Bcl-2 was decreased by AS-ODN, and the reduction of protein expression in cells treated with GA(1)-containing liposomes was more remarkable than that with GM(1a)-containing liposomes. Furthermore, the induction rate of apoptosis was higher in cells treated with AS-ODN with GA(1)-containing liposomes. Together with the results obtained by luciferase assay mentioned above, the removal of sialic acid from ganglioside causes the enhancement of efficiency of transfection mediated by cationic liposomes. |
| | |
Authors:
|
Shoichiro Noguchi; Naohide Hirashima; Mamoru Nakanishi |
Related Documents
:
|
2102776 - Bone marrow and intrathymic precursors of t-cells produce a factor which enhances colon... 3494996 - Differential regulation of colony-stimulating factors and interleukin 2 production by c... 8704276 - Granulocyte colony-stimulating factor receptor mediated cell growth and differentiation. 9095406 - Intramarrow cytokine gene transfer by adenoviral vectors in dogs. 22058116 - Protumoral role of monocytes in human b-cell precursor acute lymphoblastic leukemia: in... 9544166 - Donor lymphocyte infusions to displace residual host hematopoietic cells after allogene... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Biological & pharmaceutical bulletin Volume: 26 ISSN: 0918-6158 ISO Abbreviation: Biol. Pharm. Bull. Publication Date: 2003 Sep |
Date Detail:
|
Created Date: 2003-09-02 Completed Date: 2004-04-09 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 9311984 Medline TA: Biol Pharm Bull Country: Japan |
Other Details:
|
Languages: eng Pagination: 1306-10 Citation Subset: IM |
Affiliation:
|
Graduate School of Pharmaceutical Sciences, Nagoya City University, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
drug effects Blotting, Western Cations Cell Line, Tumor Cholesterol / chemistry* Drug Carriers* Galactose / chemistry Gene Transfer Techniques Genes, bcl-2 / genetics Glycosphingolipids / chemistry, pharmacology* Hela Cells Humans Liposomes* Luciferases / genetics Oligonucleotides, Antisense / administration & dosage Transfection* |
| Chemical | |
Reg. No./Substance:
|
0/Cations; 0/Drug Carriers; 0/Glycosphingolipids; 0/Liposomes; 0/Oligonucleotides, Antisense; 26566-61-0/Galactose; 57-88-5/Cholesterol; EC 1.13.12.-/Luciferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Preparation and rectal absorption of highly concentrated glycyrrhizin solution.
Next Document: Age dependency of esterase activity in rat and human keratinocytes.