Document Detail


Asialoerythropoietin has strong renoprotective effects against ischemia-reperfusion injury in a murine model.
MedLine Citation:
PMID:  17713435     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The renoprotective effect of erythropoietin (EPO) and the nonhematopoietic EPO, asialoEPO was investigated in a murine ischemia-reperfusion injury (I/R) model. METHODS: I/R was created by clamping the right renal pedicle for 60 min after left nephrectomy. Balb/c mice were divided into four groups (n=15 in each group): sham operation (Sham), vehicle treatment (Vehicle), EPO treatment (EPO), and asialoEPO treatment (AsialoEPO). EPO and asialoEPO were given at a dose of 500 IU/kg 30 min before I/R. Plasma creatinine (Cr), survival, and the number of apoptotic cells were analyzed. Protein expression was analyzed by Western blotting. RESULTS: Plasma Cr level was not significantly different at 6 hr after I/R. At 24 hr after I/R, the Cr (mg/dL) levels in Sham, Vehicle, EPO, and asialoEPO were 0.13+/-0.01, 1.24+/-0.70, 0.24+/-0.08, and 0.25+/-0.13, respectively (P<0.05). The numbers of apoptotic cells in these groups were 0.1+/-0.1, 98.9+/-42.6, 3.3+/-0.7, and 2.9+/-1.6, respectively (P<0.05). Western blotting revealed that in kidney tissue of mice treated with EPO and asialoEPO, p38-MAPK and the proapoptotic molecule Bad was decreased, and the antiapoptotic molecules Bcl-xL and XIAP were increased. Survival rates at 7 days after I/R injury in the Sham, Vehicle, EPO, and AsialoEPO groups were 100%, 21.4%, 23.1%, and 53.8%, respectively (P=0.05). CONCLUSION: EPO and asialoEPO attenuated renal dysfunction caused by I/R in mouse kidney at the same level, but only asialoEPO improved survival.
Authors:
Toshie Okada; Tokihiko Sawada; Keiichi Kubota
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transplantation     Volume:  84     ISSN:  0041-1337     ISO Abbreviation:  Transplantation     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-08-23     Completed Date:  2007-10-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0132144     Medline TA:  Transplantation     Country:  United States    
Other Details:
Languages:  eng     Pagination:  504-10     Citation Subset:  IM    
Affiliation:
Second Department of Surgery, Dokkyo University School of Medicine, Kitakobayashi 880, Mibu, Shimotsuga 880, Tochigi 321-0293, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects
Asialoglycoproteins / therapeutic use*
Creatinine / blood
Disease Models, Animal
Erythropoietin / analogs & derivatives*,  therapeutic use
Female
Kidney / metabolism,  pathology,  physiopathology*
Mice
Mice, Inbred BALB C
Random Allocation
Reperfusion Injury / metabolism,  pathology,  prevention & control*
Survival Rate
X-Linked Inhibitor of Apoptosis Protein / metabolism
bcl-Associated Death Protein / antagonists & inhibitors
bcl-X Protein / metabolism
p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors
Chemical
Reg. No./Substance:
0/Asialoglycoproteins; 0/Bad protein, mouse; 0/Bcl2l1 protein, mouse; 0/X-Linked Inhibitor of Apoptosis Protein; 0/asialoerythropoietin; 0/bcl-Associated Death Protein; 0/bcl-X Protein; 11096-26-7/Erythropoietin; 60-27-5/Creatinine; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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