Document Detail


Asexual cephalosporin C producer Acremonium chrysogenum carries a functional mating type locus.
MedLine Citation:
PMID:  18689517     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Acremonium chrysogenum, the fungal producer of the pharmaceutically relevant beta-lactam antibiotic cephalosporin C, is classified as asexual because no direct observation of mating or meiosis has yet been reported. To assess the potential of A. chrysogenum for sexual reproduction, we screened an expressed sequence tag library from A. chrysogenum for the expression of mating type (MAT) genes, which are the key regulators of sexual reproduction. We identified two putative mating type genes that are homologues of the alpha-box domain gene, MAT1-1-1 and MAT1-1-2, encoding an HPG domain protein defined by the presence of the three invariant amino acids histidine, proline, and glycine. In addition, cDNAs encoding a putative pheromone receptor and pheromone-processing enzymes, as well as components of a pheromone response pathway, were found. Moreover, the entire A. chrysogenum MAT1-1 (AcMAT1-1) gene and regions flanking the MAT region were obtained from a genomic cosmid library, and sequence analysis revealed that in addition to AcMAT1-1-1 and AcMAT1-1-2, the AcMAT1-1 locus comprises a third mating type gene, AcMAT1-1-3, encoding a high-mobility-group domain protein. The alpha-box domain sequence of AcMAT1-1-1 was used to determine the phylogenetic relationships of A. chrysogenum to other ascomycetes. To determine the functionality of the AcMAT1-1 locus, the entire MAT locus was transferred into a MAT deletion strain of the heterothallic ascomycete Podospora anserina (the PaDeltaMAT strain). After fertilization with a P. anserina MAT1-2 (MAT(+)) strain, the corresponding transformants developed fruiting bodies with mature ascospores. Thus, the results of our functional analysis of the AcMAT1-1 locus provide strong evidence to hypothesize a sexual cycle in A. chrysogenum.
Authors:
Stefanie Pöggeler; Birgit Hoff; Ulrich Kück
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-08
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  74     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-26     Completed Date:  2008-10-23     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6006-16     Citation Subset:  IM    
Affiliation:
Abteilung Genetik Eukaryotischer Mikroorganismen, Institut für Mikrobiologie und Genetik, Georg-August Universität Göttingen,Grisebachstr. 8, 37077 Göttingen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Acremonium / physiology*
Amino Acid Sequence
DNA, Fungal / chemistry,  genetics
Expressed Sequence Tags
Fungal Proteins / genetics
Gene Library
Gene Order
Genes, Mating Type, Fungal*
Molecular Sequence Data
Phylogeny
Podospora / cytology,  genetics
Receptors, Pheromone / genetics
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology
Spores, Fungal / cytology
Chemical
Reg. No./Substance:
0/DNA, Fungal; 0/Fungal Proteins; 0/Receptors, Pheromone
Comments/Corrections

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