Document Detail

Ascorbic and dehydroascorbic acids measured in plasma preserved with dithiothreitol or metaphosphoric acid.
MedLine Citation:
PMID:  2208649     Owner:  NLM     Status:  MEDLINE    
We describe a rapid method for accurately and precisely measuring ascorbic acid and dehydroascorbic acid in plasma. Total analysis time is less than 10 min, replicate analyses of a single pool provide precision less than or equal to 2%, and values measured in supplemented samples agree with known concentrations of 4.68 and 11.83 mg/L. The stability and homogeneity of lyophilized plasma samples supplemented with ascorbic acid and dithiothreitol are documented. We also describe a procedure in which metaphosphoric acid (50 g/L) is used to prepare a reference material for the measurement of ascorbic acid and dehydroascorbic acid. The procedure for both acids consists of first measuring the native ascorbic acid, then reducing the dehydroascorbic acid, at neutral pH, with dithiothreitol, and finally measuring the total ascorbic acid; dehydroascorbic acid is then determined by difference. The metaphosphoric-acid-treated samples were stable at -70 degrees C, but stability decreased with temperature over the range examined, 4-50 degrees C.
S A Margolis; R C Paule; R G Ziegler
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Clinical chemistry     Volume:  36     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1990 Oct 
Date Detail:
Created Date:  1990-11-21     Completed Date:  1990-11-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1750-5     Citation Subset:  IM    
Organic Analytical Chemistry Division, National Institute for Standards and Technology, Gaithersburg, MD 20899.
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MeSH Terms
Ascorbic Acid / blood*,  standards
Blood Preservation* / methods
Dehydroascorbic Acid / blood*,  standards
Drug Stability
Phosphorous Acids
Reproducibility of Results
Grant Support
Reg. No./Substance:
0/Phosphorous Acids; 10343-62-1/metaphosphoric acid; 3483-12-3/Dithiothreitol; 490-83-5/Dehydroascorbic Acid; 50-81-7/Ascorbic Acid

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