Document Detail

Ascorbic acid and uric acid suppress glucose-induced fibronectin and vascular endothelial growth factor production in human endothelial cells.
MedLine Citation:
PMID:  15745839     Owner:  NLM     Status:  MEDLINE    
Human umbilical vein endothelial cells (HUVECs) were used to investigate the individual effect of ascorbic (0.01 and 0.05 mM) and uric acid (3, 5, and 7 mg/dl), as well as the combined effect of these two agents on glucose-induced overexpression of fibronectin (Fn), glutathione peroxidase (GPx) activity, H2O2 level and vascular endothelial growth factor (VEGF) protein levels. Under 30 mM glucose stress, GPx activity significantly decreased, and lactate dehydrogenase (LDH) release, H2O2 level, and Fn and VEGF production significantly increased in HUVECs (P<.05). The addition of ascorbic acid at 0.05 mM or uric acid at 5 and 7 mg/dl significantly reduced LDH release, recovered GPx activity, suppressed H2O2 production, and decreased Fn and VEGF production (P<.05). The combination of 0.05 mM ascorbic acid and 3, 5, or 7 mg/dl provided significantly greater effect in enhancing GPx activity and lowering H2O2 level than ascorbic acid or uric acid treatment alone did (P<.05). These data suggest that ascorbic acid alone or combined with uric acid in these concentrations may be beneficial to ameliorate glucose-induced diabetic deterioration.
Cheng-Chin Hsu; Mei-Chin Yin; Ruling Tian
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of diabetes and its complications     Volume:  19     ISSN:  1056-8727     ISO Abbreviation:  J. Diabetes Complicat.     Publication Date:    2005 Mar-Apr
Date Detail:
Created Date:  2005-03-04     Completed Date:  2005-06-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9204583     Medline TA:  J Diabetes Complications     Country:  United States    
Other Details:
Languages:  eng     Pagination:  96-100     Citation Subset:  IM    
Department of Nutritional Science, Chungshan Medical University, Number 110, Section 1, Chien-Kuo North Road, Taichung City, Taiwan, ROC.
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MeSH Terms
Ascorbic Acid / pharmacology*
Cells, Cultured
Endothelium, Vascular / drug effects,  physiology*
Fibronectins / metabolism*
Glucose / pharmacology
Glutathione Peroxidase / metabolism
Hydrogen Peroxide / metabolism
L-Lactate Dehydrogenase / metabolism
Umbilical Veins
Uric Acid / pharmacology*
Vascular Endothelial Growth Factor A / metabolism*
Reg. No./Substance:
0/Fibronectins; 0/Vascular Endothelial Growth Factor A; 50-81-7/Ascorbic Acid; 50-99-7/Glucose; 69-93-2/Uric Acid; 7722-84-1/Hydrogen Peroxide; EC Dehydrogenase; EC Peroxidase

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