| Ascorbic acid contents in transgenic potato plants overexpressing two dehydroascorbate reductase genes. | |
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MedLine Citation:
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PMID: 20857222 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Ascorbic acid (AsA, vitamin C) is one of the most important nutritional quality factors in many horticultural crops and has many biological activities in the human body. Dehydroascorbate reductase (EC 1.8.5.1; DHAR) plays an important role in maintaining the normal level of ascorbic acid (AsA) by recycling oxidized ascorbic acid. To increase AsA content of potato, we isolated and characterized the cDNAs encoding two isoform DHARs localized in cytosol and chloroplast from potato, and developed two types of transgenic potato plants overexpressing cytosolic DHAR gene and chloroplastic DHAR, respectively. Incorporation of the transgene in the genome of potato was confirmed by PCR and real time RT-PCR. The overexpression of cytosolic DHAR significantly increased DHAR activities and AsA contents in potato leaves and tubers, whereas chloroplastic DHAR overexpression only increased DHAR activities and AsA contents in leaves, and did not change them in tubers. These results indicated that AsA content of potato can be elevated by enhancing recycling ascorbate via DHAR overexpression, moreover, cytosolic DHAR might play main important roles in improving the AsA contents of potato tubers. |
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Authors:
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Aiguo Qin; Qinghua Shi; Xianchang Yu |
Publication Detail:
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Type: Journal Article Date: 2010-09-21 |
Journal Detail:
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Title: Molecular biology reports Volume: 38 ISSN: 1573-4978 ISO Abbreviation: Mol. Biol. Rep. Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-02-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0403234 Medline TA: Mol Biol Rep Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1557-66 Citation Subset: IM |
Affiliation:
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Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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