Document Detail

Ascorbic acid and catecholamine release from digitonin-treated chromaffin cells.
MedLine Citation:
PMID:  4066665     Owner:  NLM     Status:  MEDLINE    
The subcellular localization of catecholamines and ascorbic acid in cultured bovine adrenal chromaffin cells was studied by permeabilizing the cells with digitonin, a steroid glycoside. Catecholamine release from permeabilized chromaffin cells was dependent on the free calcium concentration and the temperature of the incubation mixture. By contrast, [14C]ascorbic acid, preloaded into the cells, was released by digitonin treatment in a manner independent of the concentration of free calcium and with only moderate regard to the incubation temperature. The sensitivity of ascorbic acid release to digitonin treatment was identical to that of calcium-dependent catecholamine release. These results thus suggest that ascorbic acid preloaded into the cells may directly efflux from the cell cytoplasm as a result of the permeabilization of the plasma membrane. Dimethylepinephrine, a permanently positively charged catecholamine analog which is known to be excluded from vesicular fractions, was also released by digitonin treatment in a manner independent of calcium. The time course of dimethylepinephrine release was very similar to that of ascorbic acid release. Thus, newly accumulated ascorbic acid in chromaffin cells may be localized to a free pool in the cell cytoplasm rather than in a vesicular compartment.
K Morita; M Levine; E Heldman; H B Pollard
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  260     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1985 Dec 
Date Detail:
Created Date:  1986-01-03     Completed Date:  1986-01-03     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  15112-6     Citation Subset:  IM    
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MeSH Terms
Adrenal Medulla / cytology
Ascorbic Acid / metabolism*
Calcium / metabolism
Catecholamines / metabolism*
Cell Membrane Permeability / drug effects
Cells, Cultured
Chromaffin Granules / drug effects,  metabolism*
Chromaffin System / metabolism*
Digitonin / pharmacology*
Dopamine beta-Hydroxylase / metabolism
Epinephrine / analogs & derivatives,  metabolism
Reg. No./Substance:
0/Catecholamines; 11024-24-1/Digitonin; 38522-73-5/N,N-dimethylepinephrine; 50-81-7/Ascorbic Acid; 51-43-4/Epinephrine; 7440-70-2/Calcium; EC beta-Hydroxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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