Document Detail


Ascorbic acid 2-O-alpha-glucoside, a stable form of ascorbic acid, rescues human keratinocyte cell line, SCC, from cytotoxicity of ultraviolet light B.
MedLine Citation:
PMID:  8839974     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Environmental exposure to ultraviolet light B (UVB, wave lengths 290-320 nm) of the solar spectrum causes major damage, including an inflammatory response, in skin. In the present study, we estimated the ability of a stable derivative of ascorbic acid, ascorbic acid 2-O-alpha-glucoside (AA-2G), to reduce UVB damage, using the human keratinocyte cell line, SCC, established from squamous cell carcinoma. By pre- (9 h) and post-cultivation with AA-2G, a significant preventive effect on the decrease in the absolute number of surviving cells by exposure to UVB (typical dose, 20 mJ/cm2) was measurable by a neutral red-uptake assay. The release of lactate dehydrogenase from the cell membrane damaged by UVB was inhibited by AA-2G. In agarose gel electrophoresis, relatively high molecular weight DNA fragments were detected in irradiated cells after 6 h post-irradiation, suggesting that the mechanism of cell death was necrosis. Quantitative analysis of DNA content by flow cytometry indicated that AA-2G suppressed both an increase in debris with degraded nuclei and a decrease in cells in G1 and S phases, but not in the G2/M phase, by UVB exposure. These data suggest that AA-2G shows a photoprotective effect against UVB-induced damage in human epithelial cells.
Authors:
E Miyai; M Yanagida; J Akiyama; I Yamamoto
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  19     ISSN:  0918-6158     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1997-01-30     Completed Date:  1997-01-30     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  984-7     Citation Subset:  IM    
Affiliation:
Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Ascorbic Acid / analogs & derivatives*,  pharmacology
Carcinoma, Squamous Cell
Cell Death / drug effects,  radiation effects
DNA / biosynthesis,  drug effects,  radiation effects
Flow Cytometry
Humans
Keratinocytes / drug effects*,  radiation effects*
L-Lactate Dehydrogenase / metabolism
Radiation-Protective Agents / pharmacology*
Skin Neoplasms
Tumor Cells, Cultured
Ultraviolet Rays
Chemical
Reg. No./Substance:
0/Radiation-Protective Agents; 129499-78-1/ascorbic acid 2-O-glucoside; 50-81-7/Ascorbic Acid; 9007-49-2/DNA; EC 1.1.1.27/L-Lactate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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