| Ascorbic acid improves embryonic cardiomyoblast cell survival and promotes vascularization in potential myocardial grafts in vivo. | |
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MedLine Citation:
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PMID: 19908964 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Organ restoration via cell therapy and tissue transplantation is limited by impaired graft survival. We tested the hypothesis that ascorbic acid (AA) reduces cell death in myocardial grafts both in vitro and in vivo and introduced a new model of autologous graft vascularization for later transplantation. Luciferase (Fluc)- and green fluorescent protein (GFP)-expressing H9C2 cardiomyoblasts were seeded in gelatin scaffolds to form myocardial artificial grafts (MAGs). MAGs were supplemented with AA (5 or 50 mumol/L) or plain growth medium. Bioluminescence imaging showed increased cell photon emission from day 1 to 5 in grafts supplemented with 5 mumol/L (p < 0.001) and 50 mumol/L (p < 0.01) AA. The amount of apoptotic cells in plain MAGs was significantly higher than in AA-enriched grafts. In our in vitro model, AA also enhanced H9C2 cell myogenic differentiation. For in vivo studies, MAGs containing H9C2-GFP-Fluc cells and enriched with AA (n = 10) or phosphate-buffered saline (n = 10) were implanted in the renal pouch of Wistar rats. At day 6, postimplantation bioluminescence signals decreased by 74% of baseline in plain MAGs versus 36% in AA-enriched MAGs (p < 0.0001). AA grafts contained significantly higher amounts of blood vessels, GFP(+) donor cells, and endothelial cells. In this study, we identified AA as a potent supplement that improves cardiomyoblast survival and promotes neovascularization in bioartificial grafts. |
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Authors:
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Eliana C Martinez; Jing Wang; Shu Uin Gan; Rajeev Singh; Chuen Neng Lee; Theo Kofidis |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Tissue engineering. Part A Volume: 16 ISSN: 1937-335X ISO Abbreviation: Tissue Eng Part A Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-06 Completed Date: 2010-07-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101466659 Medline TA: Tissue Eng Part A Country: United States |
Other Details:
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Languages: eng Pagination: 1349-61 Citation Subset: IM |
Affiliation:
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Department of Surgery, National University of Singapore , Singapore. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Ascorbic Acid / pharmacology* Cell Line Cell Survival / drug effects Embryonic Stem Cells / cytology, drug effects*, transplantation Graft Survival / drug effects Green Fluorescent Proteins / metabolism Male Myoblasts, Cardiac / cytology, drug effects*, transplantation Myocardium / cytology* Neovascularization, Physiologic / drug effects Phenotype Rats Rats, Wistar Recombinant Proteins / metabolism Tissue Engineering / methods* Tissue Scaffolds |
| Chemical | |
Reg. No./Substance:
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0/Recombinant Proteins; 147336-22-9/Green Fluorescent Proteins; 50-81-7/Ascorbic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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