Document Detail


Ascorbic acid improves embryonic cardiomyoblast cell survival and promotes vascularization in potential myocardial grafts in vivo.
MedLine Citation:
PMID:  19908964     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Organ restoration via cell therapy and tissue transplantation is limited by impaired graft survival. We tested the hypothesis that ascorbic acid (AA) reduces cell death in myocardial grafts both in vitro and in vivo and introduced a new model of autologous graft vascularization for later transplantation. Luciferase (Fluc)- and green fluorescent protein (GFP)-expressing H9C2 cardiomyoblasts were seeded in gelatin scaffolds to form myocardial artificial grafts (MAGs). MAGs were supplemented with AA (5 or 50 mumol/L) or plain growth medium. Bioluminescence imaging showed increased cell photon emission from day 1 to 5 in grafts supplemented with 5 mumol/L (p < 0.001) and 50 mumol/L (p < 0.01) AA. The amount of apoptotic cells in plain MAGs was significantly higher than in AA-enriched grafts. In our in vitro model, AA also enhanced H9C2 cell myogenic differentiation. For in vivo studies, MAGs containing H9C2-GFP-Fluc cells and enriched with AA (n = 10) or phosphate-buffered saline (n = 10) were implanted in the renal pouch of Wistar rats. At day 6, postimplantation bioluminescence signals decreased by 74% of baseline in plain MAGs versus 36% in AA-enriched MAGs (p < 0.0001). AA grafts contained significantly higher amounts of blood vessels, GFP(+) donor cells, and endothelial cells. In this study, we identified AA as a potent supplement that improves cardiomyoblast survival and promotes neovascularization in bioartificial grafts.
Authors:
Eliana C Martinez; Jing Wang; Shu Uin Gan; Rajeev Singh; Chuen Neng Lee; Theo Kofidis
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  16     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-06     Completed Date:  2010-07-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1349-61     Citation Subset:  IM    
Affiliation:
Department of Surgery, National University of Singapore , Singapore.
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MeSH Terms
Descriptor/Qualifier:
Animals
Ascorbic Acid / pharmacology*
Cell Line
Cell Survival / drug effects
Embryonic Stem Cells / cytology,  drug effects*,  transplantation
Graft Survival / drug effects
Green Fluorescent Proteins / metabolism
Male
Myoblasts, Cardiac / cytology,  drug effects*,  transplantation
Myocardium / cytology*
Neovascularization, Physiologic / drug effects
Phenotype
Rats
Rats, Wistar
Recombinant Proteins / metabolism
Tissue Engineering / methods*
Tissue Scaffolds
Chemical
Reg. No./Substance:
0/Recombinant Proteins; 147336-22-9/Green Fluorescent Proteins; 50-81-7/Ascorbic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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