Document Detail

Ascorbate stimulates type I and type III collagen in human Tenon's fibroblasts.
MedLine Citation:
PMID:  9407369     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To better understand wound healing after glaucoma filtration surgery by measuring the production of type I and type III collagen in cultured Tenon's fibroblasts and determine the effect of ascorbic acid on collagen subtype production. METHODS: An ELISA-type dot blot assay was used to directly measure the production of types I and III collagen by subconfluent cultures of fibroblasts from human Tenon's capsule. Because ascorbic acid is both high in aqueous humor and necessary for the production of collagen, we measured the dose response of type I and type III collagen production to ascorbic acid. RESULTS: Ascorbic acid stimulated an increase in collagen production that reached a maximum level at 100 micrograms/ml. This is approximately half of the ascorbic acid concentration found in human aqueous humor. Unlike previous reports, we found no toxic effects from ascorbic acid at concentrations as high as 250 micrograms/ml over a 24-hour period. The lack of toxicity may result from the use of serum-free media in the assay. CONCLUSIONS: This culture system will be useful for exploring factors that may alter collagen production and could potentially affect wound healing.
M D Wendt; C N Soparkar; K Louie; S F Basinger; R L Gross
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of glaucoma     Volume:  6     ISSN:  1057-0829     ISO Abbreviation:  J. Glaucoma     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-01-16     Completed Date:  1998-01-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9300903     Medline TA:  J Glaucoma     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  402-7     Citation Subset:  IM    
Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, Houston, TX 77030, USA.
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MeSH Terms
Ascorbic Acid / pharmacology*
Cell Count
Cell Culture Techniques
Cell Division
Cell Survival
Collagen / biosynthesis*
Connective Tissue Cells / drug effects,  metabolism
Dose-Response Relationship, Drug
Enzyme-Linked Immunosorbent Assay
Eye / drug effects*,  metabolism
Fibroblasts / drug effects*,  metabolism
Reg. No./Substance:
50-81-7/Ascorbic Acid; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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