| Ascorbate in the guinea pig lens: dependence on drinking water supplementation. | |
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MedLine Citation:
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PMID: 15799738 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To investigate whether lens ascorbate concentration can be elevated with drinking water supplementation. METHODS: Pigmented guinea pigs received drinking water supplemented with L-ascorbate, concentration 0.00, 2.84, 5.68 or 8.52 mm for a duration of 4 weeks. In addition, the chow fed to all animals contained 125 mmol L-ascorbate per kg of chow. At the end of the supplementation period, the guinea pigs were killed. Each lens was extracted. The lens was processed and ascorbate concentration was measured using high performance liquid chromatography (HPLC) with 254 nm ultraviolet radiation detection. The data were analysed with regression. RESULTS: At the end of the test period, all lenses were devoid of cataract as observed by slit-lamp examination. All lenses contained a detectable concentration of ascorbate. Estimated 95% confidence intervals for mean animal-averaged lens ascorbate concentrations (micromol/g wet weight of whole lens) per group were 0.51 +/- 0.04 (0.00 mm; n = 6), 0.70 +/- 0.18 (2.84 mm; n = 6), 0.71 +/- 0.11 (5.68 mm; n = 5), and 0.71 +/- 0.06 (8.52 mm; n = 6). Animal-averaged lens ascorbate concentration [Asc(lens)] (micromol/g wet weight lens) increased with ascorbate supplementation in drinking water [Asc(water)] (M), in agreement with the model: [Asc(lens)] = A - Be(-kAsc(water)]. CONCLUSION: Lens ascorbate concentration increases with drinking water supplementation in the guinea pig without cataract development. The currently presented method for measurement of whole lens ascorbate content is suitable. |
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Authors:
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Vino C Mody; Manoj Kakar; Ase Elfving; Per G Söderberg; Stefan Löfgren |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta ophthalmologica Scandinavica Volume: 83 ISSN: 1395-3907 ISO Abbreviation: Acta Ophthalmol Scand Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-03-31 Completed Date: 2005-05-26 Revised Date: 2008-03-17 |
Medline Journal Info:
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Nlm Unique ID: 9507578 Medline TA: Acta Ophthalmol Scand Country: Denmark |
Other Details:
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Languages: eng Pagination: 228-33 Citation Subset: IM |
Affiliation:
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St. Erik's Eye Hospital, Karolinska Institute, Stockholm, Sweden. vino.mody@ste.ki.se |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Oral Animals Ascorbic Acid / administration & dosage*, metabolism* Chromatography, High Pressure Liquid Drinking Guinea Pigs Lens, Crystalline / metabolism* |
| Chemical | |
Reg. No./Substance:
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50-81-7/Ascorbic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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