Document Detail


Ascorbate and dehydroascorbate measurements in aqueous solutions and plasma determined by gas chromatography-mass spectrometry.
MedLine Citation:
PMID:  8439008     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The tert-butyldlmethylsllyl derivatives of ascorbic acid and dehydroascorbic acid were characterized by gas chromatography-mass spectrometry, and an isotope dilution assay for ascorbate and dehydroascorbate was developed using [13C6]ascorbic acid and [13C6]- and [6,6-2H2]dehydroascorbate. This assay was used to monitor ascorbic acid loss and the resulting rise of dehydroascorbic acid in aqueous solutions and plasma. Ascorbic acid was shown to rapidly decompose in aqueous solutions containing transition metal ions or when exposed to oxygen. Ethylenedlaminetetraacetic acid chelation did not prevent ascorbic acid degradation in aqueous solution, and ascorbate in ethylenedlaminetetraacetic acid chelated plasma was converted to dehydroascorbate on freezing. Gas chromatography-mass spectrometry appears to be a satisfactory method for determining the ascorbate and dehydroascorbate content of solutions including human blood plasma, whether or not there is ongoing oxidation of ascorbate in those solutions.
Authors:
J C Deutsch; J F Kolhouse
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical chemistry     Volume:  65     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-03-23     Completed Date:  1993-03-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  321-6     Citation Subset:  IM    
Affiliation:
Department of Medicine, Department of Veterans Affairs Hospital, Denver, Colorado.
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MeSH Terms
Descriptor/Qualifier:
Ascorbic Acid / analysis,  blood*
Dehydroascorbic Acid / analysis,  blood*
Edetic Acid
Gas Chromatography-Mass Spectrometry*
Humans
Metals
Oxidation-Reduction
Grant Support
ID/Acronym/Agency:
GM26486/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Metals; 490-83-5/Dehydroascorbic Acid; 50-81-7/Ascorbic Acid; 60-00-4/Edetic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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