| Ascorbate-2-sulfate sulfohydrolase in fish and mammal. Comparative characterization and possible involvement in ascorbate metabolism. | |
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MedLine Citation:
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PMID: 8472539 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. The new assay conditions were determined for crude and purified enzyme ascorbate-2-sulfate sulfohydrolase from liver tissues of two fish species and bovine. 2. The major departure from the existing indirect method, based on reduction of 2,6-dichlorophenolindophenol (DCIP) by released ascorbic acid and change from pink-blue to a colorless molecule, takes into account the shift of maximum absorbance of DCIP from 516 nm at pH 5.14 to 600 nm at pH 6.5. 3. The direct method is based on colorimetric assay of liberated ascorbic acid including correction for interfering substances. The optimum pH for both fish ascorbate sulfatases was 5.5. 4. The Km for bovine ascorbate sulfatase was confirmed to be approximately 7 mM at 37 degrees C. 5. Partly purified ascorbate-sulfate sulfohydrolase has a Km value in rainbow trout of 0.4 mM and it changes very little in the range of water temperatures characteristic for this stenothermic fish species. 6. In eurythermic chub, the Km values increased from 1.2 to 4.3 mM with rising temperatures. |
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Authors:
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K Dabrowski; R Lackner; C Doblander |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Comparative biochemistry and physiology. B, Comparative biochemistry Volume: 104 ISSN: 0305-0491 ISO Abbreviation: Comp. Biochem. Physiol., B Publication Date: 1993 Apr |
Date Detail:
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Created Date: 1993-05-17 Completed Date: 1993-05-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2984730R Medline TA: Comp Biochem Physiol B Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 717-22 Citation Subset: IM |
Affiliation:
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Institute of Zoology, University of Innsbruck, Austria. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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2,6-Dichloroindophenol
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metabolism Animals Arylsulfatases / metabolism* Ascorbic Acid / metabolism* Cattle Fishes / metabolism* Hydrogen-Ion Concentration Kinetics Liver / enzymology Oxidation-Reduction Spectrophotometry Trout / metabolism* |
| Chemical | |
Reg. No./Substance:
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50-81-7/Ascorbic Acid; 956-48-9/2,6-Dichloroindophenol; EC 3.1.6.-/L-ascorbate-2-sulfate sulfohydrolase; EC 3.1.6.1/Arylsulfatases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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