Document Detail


Ascomycetous yeast species recovered from grapes damaged by honeydew and sour rot.
MedLine Citation:
PMID:  17976167     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: To identify ascomycetous yeasts recovered from sound and damaged grapes by the presence of honeydew or sour rot. METHODS AND RESULTS: In sound grapes, the mean yeast counts ranged from 3.20 +/- 1.04 log CFU g(-1) to 5.87 +/- 0.64 log CFU g(-1). In honeydew grapes, the mean counts ranged from 3.88 +/- 0.80 log CFU g(-1) to 6.64 +/- 0.77 log CFU g(-1). In sour rot grapes counts varied between 6.34 +/- 1.03 and 7.68 +/- 0.38 logCFU g(-1). Hanseniaspora uvarum was the most frequent species from sound samples. In both types of damage, the most frequent species were Candida vanderwaltii, H. uvarum and Zygoascus hellenicus. The latter species was recovered in high frequency because of the utilization of the selective medium DBDM (Dekkera/Brettanomyces differential medium). The scarce isolation frequency of the wine spoilage species Zygosaccharomyces bailii (in sour rotten grapes) and Zygosaccharomyces bisporus (in honeydew affected grapes) could only be demonstrated by the use of the selective medium ZDM (Zygosaccharomyces differential medium). CONCLUSIONS: The isolation of several species only from damaged grapes indicates that damage constituted the main factor determining yeast diversity. The utilization of selective media is required for eliciting the recovery of potentially wine spoilage species. SIGNIFICANCE AND IMPACT OF THE STUDY: The impact of damaged grapes in the yeast ecology of grapes has been underestimated.
Authors:
A Barata; F Seborro; C Belloch; M Malfeito-Ferreira; V Loureiro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-01
Journal Detail:
Title:  Journal of applied microbiology     Volume:  104     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-14     Completed Date:  2008-07-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1182-91     Citation Subset:  IM    
Affiliation:
Laboratório de Microbiologia, Departamento de Botânica e Engenharia Biológica, Instituto Superior de Agronomia, Technical University of Lisbon, Lisboa, Portugal.
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MeSH Terms
Descriptor/Qualifier:
Candida / isolation & purification
Ecosystem
Food Microbiology*
Genes, Fungal
Kluyveromyces / isolation & purification
Mycological Typing Techniques
Mycoses / microbiology*
Plant Diseases / microbiology*
Polymorphism, Restriction Fragment Length
Vitis / microbiology*
Yeasts / isolation & purification*
Zygosaccharomyces / isolation & purification

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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