Document Detail


Ascl3 marks adult progenitor cells of the mouse salivary gland.
MedLine Citation:
PMID:  22370009     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Ascl3 transcription factor marks a subset of salivary gland duct cells present in the three major salivary glands of the mouse. In vivo, these cells generate both duct and secretory acinar cell descendants. Here, we have analyzed whether Ascl3-expressing cells retain this multipotent lineage potential in adult glands. Cells isolated from mouse salivary glands were cultured in vitro as non-adherent spheres. Lineage tracing of the Ascl3-expressing cells within the spheres demonstrates that Ascl3+ cells isolated from adult glands remain multipotent, generating both duct and acinar cell types in vitro. Furthermore, we demonstrate that the progenitor cells characterized by Keratin 5 expression are an independent population from Ascl3+ progenitor cells. We conclude that the Ascl3+ cells are intermediate lineage-restricted progenitor cells of the adult salivary glands.
Authors:
Anastasia Rugel-Stahl; Marilyn E Elliott; Catherine E Ovitt
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-01-31
Journal Detail:
Title:  Stem cell research     Volume:  8     ISSN:  1876-7753     ISO Abbreviation:  Stem Cell Res     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-03     Completed Date:  2012-06-28     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  101316957     Medline TA:  Stem Cell Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  379-87     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Basic Helix-Loop-Helix Transcription Factors / metabolism*
Cell Lineage
Cells, Cultured
Immunohistochemistry
Keratin-5 / metabolism
Mice
Salivary Glands / cytology*
Stem Cells / cytology,  metabolism*
Grant Support
ID/Acronym/Agency:
R01 DE008921/DE/NIDCR NIH HHS; R01 DE008921/DE/NIDCR NIH HHS; R01 DE008921-23/DE/NIDCR NIH HHS; R01 DE018896/DE/NIDCR NIH HHS; R01 DE018896/DE/NIDCR NIH HHS; R01 DE018896-02/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Ascl3 protein, mouse; 0/Basic Helix-Loop-Helix Transcription Factors; 0/Keratin-5
Comments/Corrections

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