Document Detail

Ascidian sperm activating and attracting factor: importance of sulfate groups for the activities and implication of its putative receptor.
MedLine Citation:
PMID:  18789324     Owner:  NLM     Status:  MEDLINE    
The sperm activating and attracting factor (SAAF) from the eggs of the ascidian Cionaintestinalis was identified as the sulfated polyhydroxysterol,3alpha,4beta,7alpha,26-tetrahydroxy-5alpha-cholestane-3,26-disulfate. We present a functional analysis of SAAF derivatives that reveals the roles of the various SAAF functional groups. Optical isomerism does not affect SAAF activities. Hydrolysis on one side, i.e. at the sulfate groups of SAAF, decreases the sperm-activating and sperm-attracting activities, while hydrolysis on both sides resulted in the loss of both activities. Biotinylated-SAAF lost its sperm-activating ability, but retained sperm-binding and chemotactic abilities. Thus, the sulfate groups of SAAF are responsible for these activities.
Manabu Yoshida; Kogiku Shiba; Kaoru Yoshida; Hiroshi Tsuchikawa; Ouichirou Ootou; Toru Oishi; Michio Murata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-09-19
Journal Detail:
Title:  FEBS letters     Volume:  582     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-11-03     Completed Date:  2008-12-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  3429-33     Citation Subset:  IM    
Misaki Marine Biological Station, Graduate School of Science, University of Tokyo, 1024 Koajiro, Misaki, Miura 238-0225, Japan.
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MeSH Terms
Cholestanols / chemistry*,  pharmacology*
Ciona intestinalis / cytology,  physiology*
Molecular Structure
Quartz / chemistry
Receptors, Cell Surface / agonists,  physiology
Spermatozoa / drug effects,  physiology*
Structure-Activity Relationship
Sulfuric Acid Esters / chemistry*,  pharmacology*
Reg. No./Substance:
0/3,4,7,26-tetrahydroxycholestane-3,26-disulfate; 0/Cholestanols; 0/Receptors, Cell Surface; 0/Sulfuric Acid Esters; 0/egg surface sperm receptor; 14808-60-7/Quartz

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