Document Detail


Asc1 supports cell-wall integrity near bud sites by a Pkc1 independent mechanism.
MedLine Citation:
PMID:  20613984     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The yeast ribosomal protein Asc1 is a WD-protein family member. Its mammalian ortholog, RACK1 was initially discovered as a receptor for activated protein C kinase (PKC) that functions to maintain the active conformation of PKC and to support its movement to target sites. In the budding yeast though, a connection between Asc1p and the PKC signaling pathway has never been reported.
METHODOLOGY/PRINCIPAL FINDINGS: In the present study we found that asc1-deletion mutant (asc1Delta) presents some of the hallmarks of PKC signaling mutants. These include an increased sensitivity to staurosporine, a specific Pkc1p inhibitor, and susceptibility to cell-wall perturbing treatments such as hypotonic- and heat shock conditions and zymolase treatment. Microscopic analysis of asc1Delta cells revealed cell-wall invaginations near bud sites after exposure to hypotonic conditions, and the dynamic of cells' survival after this stress further supports the involvement of Asc1p in maintaining the cell-wall integrity during the mid-to late stages of bud formation. Genetic interactions between asc1 and pkc1 reveal synergistic sensitivities of a double-knock out mutant (asc1Delta/pkc1Delta) to cell-wall stress conditions, and high basal level of PKC signaling in asc1Delta. Furthermore, Asc1p has no effect on the cellular distribution or redistribution of Pkc1p at optimal or at cell-wall stress conditions.
CONCLUSIONS/SIGNIFICANCE: Taken together, our data support the idea that unlike its mammalian orthologs, Asc1p acts remotely from Pkc1p, to regulate the integrity of the cell-wall. We speculate that its role is exerted through translation regulation of bud-site related mRNAs during cells' growth.
Authors:
Daniel Melamed; Lavi Bar-Ziv; Yossi Truzman; Yoav Arava
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-30
Journal Detail:
Title:  PloS one     Volume:  5     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2010  
Date Detail:
Created Date:  2010-07-08     Completed Date:  2010-09-01     Revised Date:  2012-06-22    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e11389     Citation Subset:  IM    
Affiliation:
Department of Biology, Technion-Israel Institute of Technology, Haifa, Israel.
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MeSH Terms
Descriptor/Qualifier:
Adaptor Proteins, Signal Transducing / genetics,  physiology*
Blotting, Western
GTP-Binding Proteins / genetics,  physiology*
Protein Kinase C / genetics,  physiology*
Saccharomyces cerevisiae / growth & development,  physiology*
Saccharomyces cerevisiae Proteins / genetics,  physiology*
Signal Transduction
Chemical
Reg. No./Substance:
0/ASC1 protein, S cerevisiae; 0/Adaptor Proteins, Signal Transducing; 0/Saccharomyces cerevisiae Proteins; EC 2.7.11.13/PKC1 protein, S cerevisiae; EC 2.7.11.13/Protein Kinase C; EC 3.6.1.-/GTP-Binding Proteins
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