| Arylamine N-acetyltransferase activities in cell lines of mouse, rat, hamster and man differing in their sensitivity to 1,6-dinitropyrene. | |
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MedLine Citation:
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PMID: 2244342 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study was aimed at monitoring N-acetyltransferase activities of continuous cell lines, which differ in their sensitivity to the toxic effects of nitroaromatic compounds. Transferase activities were measured toward the acetyl acceptors sulfamethazine and p-aminobenzoic acid in partially purified preparation of cytosols. Cell lines such as hamster V79, BHK, rat hepatoma H4IIEC3G- or fibroblast 208F, which are sensitive to 1,6-dinitropyrene (1,6-DNP), possess high transferase activities ranging from 120-270 nmol/min x mg protein. In contrast, human lung cells NCI-H322, mouse and rat hepatoma cells BW1J and H5, respectively, which are resistant to 1,6-DNP contain no or low transferase activity of less than 15 nmol/min x mg. There was no apparent correlation between 1,6-DNP sensitivity and acetyltransferase levels in a few cell lines, e.g. rat hepatoma HTC, 2sFou and 5L, which express intermediate transferase activities ranging from 25-50 nmol/min x mg protein. The results suggest that acetylation is an essential step in activating 1,6-DNP to toxic products in mammalian cells. |
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Authors:
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A K Srivastava; F J Wiebel |
Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Toxicology letters Volume: 54 ISSN: 0378-4274 ISO Abbreviation: Toxicol. Lett. Publication Date: 1990 Nov |
Date Detail:
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Created Date: 1990-12-31 Completed Date: 1990-12-31 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7709027 Medline TA: Toxicol Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 71-6 Citation Subset: IM |
Affiliation:
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GSF-Institute of Toxicology, Gesellschaft für Strahlen- und Umweltforschung, Neuherberg, F.R.G. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arylamine N-Acetyltransferase / metabolism* Cell Line / drug effects Cells, Cultured Cricetinae Humans Liver Neoplasms, Experimental / enzymology Mice Pyrenes / toxicity* Rats Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Pyrenes; 42397-64-8/1,6-dinitropyrene; EC 2.3.1.5/Arylamine N-Acetyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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