| Arylacetamide deacetylase attenuates fatty-acid-induced triacylglycerol accumulation in rat hepatoma cells. | |
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MedLine Citation:
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PMID: 19654421 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mobilization of hepatic triacylglycerol stores provides substrates for mitochondrial beta-oxidation and assembly of VLDLs; however, the identity of lipolytic enzymes involved in the regulation of this process remains largely unknown. Arylacetamide deacetylase (AADA) shares homology with hormone-sensitive lipase and therefore could potentially participate in hepatic lipid metabolism, including the regulation of hepatic triacylglycerol levels. We have established McArdle-RH7777 (rat hepatoma) cell lines stably expressing mouse AADA cDNA and performed metabolic labeling as well as lipid mass analyses. Expression of AADA cDNA in McArdle-RH7777 cells significantly reduced intracellular triacylglycerol levels and apolipoprotein B secretion and increased fatty acid oxidation. These results suggest that fatty acids released by AADA-mediated hydrolysis of lipids are channeled for -oxidation rather than for the assembly of lipoproteins. |
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Authors:
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Vivien Lo; Bruce Erickson; Michaela Thomason-Hughes; Kerry W S Ko; Vernon W Dolinsky; Randy Nelson; Richard Lehner |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-08-04 |
Journal Detail:
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Title: Journal of lipid research Volume: 51 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-01-15 Completed Date: 2010-03-19 Revised Date: 2011-07-22 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
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Languages: eng Pagination: 368-77 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2S2, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carboxylic Ester Hydrolases / genetics, metabolism* Carcinoma, Hepatocellular / genetics, metabolism, pathology* Cell Line, Tumor Fatty Acids / metabolism* Gene Expression Regulation, Neoplastic Humans Intracellular Space / metabolism Lipase / metabolism Lipid Metabolism Liver / metabolism, pathology Male Mice Oxidation-Reduction Protein Transport Rats Triglycerides / metabolism*, secretion |
| Grant Support | |
ID/Acronym/Agency:
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//Canadian Institutes of Health Research |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Triglycerides; EC 3.1.1.-/Aadac protein, mouse; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.1.1.3/Lipase |
| Comments/Corrections | |
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