Document Detail


Arylacetamide deacetylase attenuates fatty-acid-induced triacylglycerol accumulation in rat hepatoma cells.
MedLine Citation:
PMID:  19654421     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mobilization of hepatic triacylglycerol stores provides substrates for mitochondrial beta-oxidation and assembly of VLDLs; however, the identity of lipolytic enzymes involved in the regulation of this process remains largely unknown. Arylacetamide deacetylase (AADA) shares homology with hormone-sensitive lipase and therefore could potentially participate in hepatic lipid metabolism, including the regulation of hepatic triacylglycerol levels. We have established McArdle-RH7777 (rat hepatoma) cell lines stably expressing mouse AADA cDNA and performed metabolic labeling as well as lipid mass analyses. Expression of AADA cDNA in McArdle-RH7777 cells significantly reduced intracellular triacylglycerol levels and apolipoprotein B secretion and increased fatty acid oxidation. These results suggest that fatty acids released by AADA-mediated hydrolysis of lipids are channeled for -oxidation rather than for the assembly of lipoproteins.
Authors:
Vivien Lo; Bruce Erickson; Michaela Thomason-Hughes; Kerry W S Ko; Vernon W Dolinsky; Randy Nelson; Richard Lehner
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-04
Journal Detail:
Title:  Journal of lipid research     Volume:  51     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-15     Completed Date:  2010-03-19     Revised Date:  2011-07-22    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  368-77     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2S2, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carboxylic Ester Hydrolases / genetics,  metabolism*
Carcinoma, Hepatocellular / genetics,  metabolism,  pathology*
Cell Line, Tumor
Fatty Acids / metabolism*
Gene Expression Regulation, Neoplastic
Humans
Intracellular Space / metabolism
Lipase / metabolism
Lipid Metabolism
Liver / metabolism,  pathology
Male
Mice
Oxidation-Reduction
Protein Transport
Rats
Triglycerides / metabolism*,  secretion
Grant Support
ID/Acronym/Agency:
//Canadian Institutes of Health Research
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Triglycerides; EC 3.1.1.-/Aadac protein, mouse; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.1.1.3/Lipase
Comments/Corrections

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