| Artificial surface-induced inflammation relies on complement factor 5: proof from a deficient person. | |
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MedLine Citation:
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PMID: 21256307 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Exposing blood to artificial surfaces results in an inflammatory response, including complement activation and cytokine release. The aim of this investigation was to study complement-dependency and independency in artificial surface-induced inflammation in human whole blood from a patient with a genetic deficiency of complement factor 5 (C5). METHODS: Whole blood from a C5-deficient patient, C5 protein reconstituted blood, and blood from a control subject was used. The complement inhibitor compstatin (C3 inhibitor) and a C5a receptor antagonist were used to block complement. Blood was circulated in closed loops of polyvinyl chloride tubing. Leukocyte CD11b expression and release of granule enzymes (myeloperoxidase, elastase, lactoferrin), cytokines (interleukins, chemokines, and growth factors; n = 27) as well as complement activation were measured after incubation. RESULTS: In C5-deficient blood, there was no formation of the terminal complement complex, as opposed to reconstituted or control blood. Release of granule enzymes was partly dependent on C3, revealed by a compstatin-dependent effect in C5-deficient blood, and partly C5a-dependent as evident from the reconstitution and control blood. The chemokines interleukin-8 and monocyte chemoattractant protein-1 were also highly complement dependent, the effect being C5a-mediated, whereas platelet-derived and vascular endothelial growth factors were partly complement dependent. Interferon-γ increased in a complement-independent manner, whereas the rest of the cytokines did not respond to the surface. Leukocyte expression of CD11b was only marginally increased in deficient blood exposed to the surface, whereas reconstitution induced a considerable, C5a-dependent increase, comparable with that of the control. CONCLUSIONS: The polyvinyl chloride surface induced a defined inflammatory response, which largely depended on C5. |
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Authors:
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Grethe Bergseth; John D Lambris; Tom Eirik Mollnes; Knut Tore Lappegård |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Annals of thoracic surgery Volume: 91 ISSN: 1552-6259 ISO Abbreviation: Ann. Thorac. Surg. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-24 Completed Date: 2011-04-11 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 15030100R Medline TA: Ann Thorac Surg Country: Netherlands |
Other Details:
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Languages: eng Pagination: 527-33 Citation Subset: AIM; IM |
Copyright Information:
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Copyright © 2011 The Society of Thoracic Surgeons. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Research Laboratory and Division of Internal Medicine, Nordland Hospital, Bodø, Norway. grethe.bergseth@nlsh.no |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biocompatible Materials
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adverse effects* Cell Degranulation / immunology Chemokines / blood Complement Activation Complement C5 / deficiency*, immunology* Cytokines / blood Environmental Exposure / adverse effects Flow Cytometry Humans Inflammation / blood*, immunology* Neutrophils / immunology |
| Grant Support | |
ID/Acronym/Agency:
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AI068730/AI/NIAID NIH HHS; EB003968/EB/NIBIB NIH HHS; GM062134/GM/NIGMS NIH HHS; P01 AI068730-04/AI/NIAID NIH HHS; R01 EB003968-04/EB/NIBIB NIH HHS; R01 GM062134-08/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Chemokines; 0/Complement C5; 0/Cytokines |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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