Document Detail

Artificial rearing of infant mice leads to n-3 fatty acid deficiency in cardiac, neural and peripheral tissues.
MedLine Citation:
PMID:  19588181     Owner:  NLM     Status:  MEDLINE    
The ability to control the fatty acid content of the diet during early development is a crucial requirement for a one-generation model of docosahexaenoic acid (DHA; 22:6n3) deficiency. A hand feeding method using artificial rearing (AR) together with sterile, artificial milk was employed for feeding mice from postnatal day 2-15. The pups were fed an n-3 fatty acid adequate (3% alpha-linolenic acid (LNA; 18:3n3) + 1% 22:6n3) or a deficient diet (0.06% 18:3n3) with linoleic acid (LA; 18:2n6) as the only dietary source of essential fatty acids by AR along with a dam-reared control group (3.1% 18:3n3). The results indicate that restriction of n-3 fatty acid intake during postnatal development leads to markedly lower levels of brain, retinal, liver, plasma and heart 22:6n3 at 20 weeks of age with replacement by docosapentaenoic acid (DPAn6; 22:5n6), arachidonic acid (ARA; 20:4n6) and docosatetraenoic acid (DTA; 22:4n6). A detailed analysis of phospholipid classes of heart tissue indicated that phosphatidylethanolamine, phosphatidylcholine and cardiolipin were the major repositories of 22:6n3, reaching 40, 29 and 15%, respectively. A novel heart cardiolipin species containing four 22:6n3 moieties is described. This is the first report of the application of artificially rearing to mouse pup nutrition; this technique will facilitate dietary studies of knockout animals as well as the study of essential fatty acid (EFA) functions in the cardiovascular, neural and other organ systems.
Nahed Hussein; Irina Fedorova; Toru Moriguchi; Kei Hamazaki; Hee-Yong Kim; Junji Hoshiba; Norman Salem
Publication Detail:
Type:  Journal Article     Date:  2009-07-09
Journal Detail:
Title:  Lipids     Volume:  44     ISSN:  1558-9307     ISO Abbreviation:  Lipids     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-23     Completed Date:  2009-10-01     Revised Date:  2010-09-27    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  685-702     Citation Subset:  IM    
Faculty of Specific Education, Ain Shams University, Cairo, Egypt.
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MeSH Terms
Animal Feed / adverse effects
Animal Husbandry / methods
Animals, Newborn
Body Weight
Deficiency Diseases / etiology*,  metabolism,  pathology
Fatty Acids, Omega-3 / metabolism*
Food, Formulated / adverse effects*
Mice, Inbred ICR
Myocardium / metabolism*,  pathology
Nervous System / metabolism*,  pathology
Organ Size
Tissue Distribution
Grant Support
Z01 AA000284-18/AA/NIAAA NIH HHS
Reg. No./Substance:
0/Fatty Acids, Omega-3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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