| Artificial rearing of infant mice leads to n-3 fatty acid deficiency in cardiac, neural and peripheral tissues. | |
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MedLine Citation:
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PMID: 19588181 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ability to control the fatty acid content of the diet during early development is a crucial requirement for a one-generation model of docosahexaenoic acid (DHA; 22:6n3) deficiency. A hand feeding method using artificial rearing (AR) together with sterile, artificial milk was employed for feeding mice from postnatal day 2-15. The pups were fed an n-3 fatty acid adequate (3% alpha-linolenic acid (LNA; 18:3n3) + 1% 22:6n3) or a deficient diet (0.06% 18:3n3) with linoleic acid (LA; 18:2n6) as the only dietary source of essential fatty acids by AR along with a dam-reared control group (3.1% 18:3n3). The results indicate that restriction of n-3 fatty acid intake during postnatal development leads to markedly lower levels of brain, retinal, liver, plasma and heart 22:6n3 at 20 weeks of age with replacement by docosapentaenoic acid (DPAn6; 22:5n6), arachidonic acid (ARA; 20:4n6) and docosatetraenoic acid (DTA; 22:4n6). A detailed analysis of phospholipid classes of heart tissue indicated that phosphatidylethanolamine, phosphatidylcholine and cardiolipin were the major repositories of 22:6n3, reaching 40, 29 and 15%, respectively. A novel heart cardiolipin species containing four 22:6n3 moieties is described. This is the first report of the application of artificially rearing to mouse pup nutrition; this technique will facilitate dietary studies of knockout animals as well as the study of essential fatty acid (EFA) functions in the cardiovascular, neural and other organ systems. |
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Authors:
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Nahed Hussein; Irina Fedorova; Toru Moriguchi; Kei Hamazaki; Hee-Yong Kim; Junji Hoshiba; Norman Salem |
Publication Detail:
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Type: Journal Article Date: 2009-07-09 |
Journal Detail:
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Title: Lipids Volume: 44 ISSN: 1558-9307 ISO Abbreviation: Lipids Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-23 Completed Date: 2009-10-01 Revised Date: 2010-09-27 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: Germany |
Other Details:
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Languages: eng Pagination: 685-702 Citation Subset: IM |
Affiliation:
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Faculty of Specific Education, Ain Shams University, Cairo, Egypt. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed
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adverse effects Animal Husbandry / methods Animals Animals, Newborn Body Weight Deficiency Diseases / etiology*, metabolism, pathology Fatty Acids, Omega-3 / metabolism* Female Food, Formulated / adverse effects* Male Mice Mice, Inbred ICR Myocardium / metabolism*, pathology Nervous System / metabolism*, pathology Organ Size Tissue Distribution |
| Grant Support | |
ID/Acronym/Agency:
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Z01 AA000284-18/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Omega-3 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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