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Artificial MicroRNAs for Specific Gene Silencing in Rice.
MedLine Citation:
PMID:  23135850     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Artificial microRNAs (amiRNAs) have been shown to facilitate efficient gene silencing with high specificity to the intended target gene(s). For the plant breeder, gene silencing by artificial miRNAs will certainly accelerate gene discovery, because it allows targeting of all genes in a mapping interval, independent of the genetic background. In addition, beneficial knockout phenotypes can easily be transferred between varieties and across incompatibility barriers. This chapter describes the generation and application of amiRNAs as a gene silencing tool in rice.
Authors:
Norman Warthmann; Stephan Ossowski; Rebecca Schwab; Detlef Weigel
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  956     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  131-49     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen, Germany, norman@warthmann.com.
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