Document Detail


Artifact formation during transmethylation of lipid peroxides.
MedLine Citation:
PMID:  1524215     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
By-product formation during base-catalyzed transesterification of lipid peroxides was observed in model experiments. A partially oxidized linoleic acid methyl ester as well as purified hydroxylinoleates served as test compounds. By-products formed during a simulated transmethylation of purified hydroxylinoleates were separated from the main components by means of thin-layer chromatography. The loss attributable to these by-products amounted to 10%. Oxygenated fatty acid derivatives were completely destroyed by acid-catalyzed transmethylation. Catalytic hydrogenation prior to base-catalyzed transmethylation proved to be a simple means to minimize side-product formation. By using this technique the yield of hydroxystearates from a partially autoxidized linoleic acid methyl ester preparation was improved significantly.
Authors:
F Ulberth; W Kamptner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  203     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-10-13     Completed Date:  1992-10-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  35-8     Citation Subset:  IM    
Affiliation:
Department of Dairy Research and Bacteriology, Agricultural University, Vienna, Austria.
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MeSH Terms
Descriptor/Qualifier:
Artifacts*
Chromatography, Thin Layer
Gas Chromatography-Mass Spectrometry
Kinetics
Linoleic Acids / chemistry
Lipid Peroxidation*
Lipid Peroxides / chemistry
Methylation
Oxidation-Reduction
Chemical
Reg. No./Substance:
0/Linoleic Acids; 0/Lipid Peroxides; 112-63-0/methyl linoleate; 25657-09-4/linoleic acid hydroperoxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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