| Articular cartilage repair using an intra-articular magnet and synovium-derived cells. | |
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MedLine Citation:
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PMID: 21064159 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The purpose of this study was to investigate the chondrogenic potential of magnetically labeled synovium-derived cells (M-SDCs) and examine whether M-SDCs could repair the articular cartilage using an intra-articular magnet after delivery to the lesion. Synovium-derived cells (SDCs) were cultured from the synovium of a rat knee, and were magnetically labeled with ferumoxides. M-SDCs were examined with a transmission electron microscope. A pellet culture system was used to evaluate the chondrogenic potential of M-SDCs in a magnetic field. In a rat model, allogeneic M-SDCs were injected into the knee after we made an osteochondral defect on the patellar groove and implanted an intra-articular magnet at the bottom of the defect. We histologically examined the defects at 48 h, 4 weeks, 8 weeks, and 12 weeks after treatment. Electron microscopy showed the transfection of ferumoxides into SDCs. The pellet cultures revealed the chondrogenic potential of M-SDCs in a magnetic field. M-SDCs accumulated in the osteochondral defect at 48 h after treatment, and we confirmed the regeneration of the articular cartilage at 4 weeks, 8 weeks, and 12 weeks after treatment using an intra-articular magnet. We demonstrated that articular cartilage defects could be repaired using an intra-articular magnet and M-SDCs. We believe that this system will be useful to repair human articular cartilage defects. © 2010 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res. |
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Authors:
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Junji Hori; Masataka Deie; Takaaki Kobayashi; Yuji Yasunaga; Seiichi Kawamata; Mitsuo Ochi |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2010-11-9 |
Journal Detail:
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Title: Journal of orthopaedic research : official publication of the Orthopaedic Research Society Volume: - ISSN: 1554-527X ISO Abbreviation: - Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8404726 Medline TA: J Orthop Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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