Document Detail


Arteriogenesis depends on circulating monocytes and macrophage accumulation and is severely depressed in op/op mice.
MedLine Citation:
PMID:  16684892     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has been suggested that monocytes/macrophages represent the pivotal cell type during early adaptive growth of pre-existent arterial anastomoses toward functional collateral arteries (arteriogenesis) upon arterial occlusion. This hypothesis was supported by previous studies providing evidence that elevation of the peripheral monocyte count, increased monocyte survival (e.g., granulocyte macrophage-colony stimulating factor), as well as enhanced attraction or adhesion (e.g., monocyte chemoattractant protein 1; intercellular adhesion molecule 1) of the latter cells correlates directly with the arteriogenic response to restore tissue perfusion. However, the experimental proof of the essential role of monocytes/macrophages remains to be given. We therefore hypothesized that arteriogenesis is reduced in a genuine, nonpharmocologically induced monocyte/macrophage-deficient model of femoral artery occlusion in osteopetrotic (op/op) mice. Total leukocyte count did not differ between op/op mice and control (B6C3Fe a/a-Csf1(+/+)) mice. op/op mice show a significant monocytopenia (0.67%+/-0.38% vs. 1.53%+/-0.32%), granulocytosis (33.66%+/-6.67% vs. 22.83+/-7.47%), and a concomitant, relative lymphopenia (65.67%+/-6.58% vs. 75.65%+/-7.31%). Microsphere-based perfusion measurement 7 days after femoral artery occlusion demonstrated a significantly reduced perfusion restoration upon femoral artery occlusion in op/op mice as compared with controls (28.19%+/-0.91% vs. 47.88%+/-2.49%). The application of a novel method of high resolution (microfocus X-ray system) angiography revealed a reduction of proliferation and diameter of collateral arteries. Quantitative immunohistology showed significantly lower numbers of macrophages in the surrounding tissue of proliferating arteries. This study provides additional evidence for the preeminent role of monocytes/macrophages during arteriogenesis in a genuine model of monocyte deficiency, i.e., without pharmacological intervention.
Authors:
Caroline E Bergmann; Imo E Hoefer; Benjamin Meder; Holger Roth; Niels van Royen; Sabine M Breit; Marco M Jost; Seyedhossein Aharinejad; Susanne Hartmann; Ivo R Buschmann
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-09
Journal Detail:
Title:  Journal of leukocyte biology     Volume:  80     ISSN:  0741-5400     ISO Abbreviation:  J. Leukoc. Biol.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-06-26     Completed Date:  2007-09-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8405628     Medline TA:  J Leukoc Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  59-65     Citation Subset:  IM    
Affiliation:
Research Group for Experimental and Clinical Arteriogenesis, Freiberg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Immunohistochemistry
Lymphocyte Count
Macrophage Colony-Stimulating Factor / blood
Macrophages / immunology*,  ultrastructure
Mice
Mice, Knockout
Models, Animal
Monocytes / immunology*,  ultrastructure
Neovascularization, Physiologic / genetics,  immunology*
Phenotype
Chemical
Reg. No./Substance:
81627-83-0/Macrophage Colony-Stimulating Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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