| Arterial remodeling and plasma volume expansion in caveolin-1-deficient mice. | |
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MedLine Citation:
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PMID: 17626133 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Caveolin-1 (Cav-1) is essential for the morphology of membrane caveolae and exerts a negative influence on a number of signaling systems, including nitric oxide (NO) production and activity of the MAP kinase cascade. In the vascular system, ablation of caveolin-1 may thus be expected to cause arterial dilatation and increased vessel wall mass (remodeling). This was tested in Cav-1 knockout (KO) mice by a detailed morphometric and functional analysis of mesenteric resistance arteries, shown to lack caveolae. Quantitative morphometry revealed increased media thickness and media-to-lumen ratio in KO. Pressure-induced myogenic tone and flow-induced dilatation were decreased in KO arteries, but both were increased toward wild-type (WT) levels following NO synthase (NOS) inhibition. Isometric force recordings following NOS inhibition showed rightward shifts of passive and active length-force relationships in KO, and the force response to alpha(1)-adrenergic stimulation was increased. In contrast, media thickness and force response of the aorta were unaltered in KO vs. WT, whereas lumen diameter was increased. Mean arterial blood pressure during isoflurane anesthesia was not different in KO vs. WT, but greater fluctuation in blood pressure over time was noted. Following NOS inhibition, fluctuations disappeared and pressure increased twice as much in KO (38 +/- 6%) compared with WT (17 +/- 3%). Tracer-dilution experiments showed increased plasma volume in KO. We conclude that NO affects blood pressure more in Cav-1 KO than in WT mice and that restructuring of resistance vessels and an increased responsiveness to adrenergic stimulation compensate for a decreased tone in Cav-1 KO mice. |
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Authors:
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Sebastian Albinsson; Yulia Shakirova; Anna Rippe; Maria Baumgarten; Bert-Inge Rosengren; Catarina Rippe; Rupert Hallmann; Per Hellstrand; Bengt Rippe; Karl Swärd |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-07-11 |
Journal Detail:
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Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: 293 ISSN: 0363-6119 ISO Abbreviation: Am. J. Physiol. Regul. Integr. Comp. Physiol. Publication Date: 2007 Sep |
Date Detail:
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Created Date: 2007-08-31 Completed Date: 2007-10-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901230 Medline TA: Am J Physiol Regul Integr Comp Physiol Country: United States |
Other Details:
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Languages: eng Pagination: R1222-31 Citation Subset: IM |
Affiliation:
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Department of Experimental Medical Science, Lund University, SE-221 84 Lund, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Pressure / physiology Blotting, Western Caveolin 1 / deficiency*, genetics Enzyme Inhibitors / pharmacology Fluorescent Antibody Technique Isometric Contraction / drug effects Kinetics Mesenteric Arteries / cytology, drug effects, physiology* Mice Mice, Inbred C57BL Mice, Knockout Microscopy, Electron, Transmission Muscle Contraction / drug effects, physiology Muscle Tonus / physiology Myography NG-Nitroarginine Methyl Ester / pharmacology Nitric Oxide Synthase Type III / antagonists & inhibitors Organ Culture Techniques Plasma Substitutes / pharmacology* Plasma Volume / drug effects, physiology* Thymidine / metabolism Vasodilation / physiology |
| Chemical | |
Reg. No./Substance:
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0/Caveolin 1; 0/Enzyme Inhibitors; 0/Plasma Substitutes; 50-89-5/Thymidine; 50903-99-6/NG-Nitroarginine Methyl Ester; EC 1.14.13.39/Nitric Oxide Synthase Type III |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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