Document Detail

Arsenic trioxide reduces the invasive and metastatic properties of nasopharyngeal carcinoma cells in vitro.
MedLine Citation:
PMID:  16648906     Owner:  NLM     Status:  MEDLINE    
Nasopharyngeal carcinoma (NPC) is notorious for the metastases, which are in close association with Epstein-Barr virus-encoded latent membrane protein 1 (LMP1). Arsenic trioxide (As2O3) has been shown to induce apoptosis and differentiation in NPC xenografts. Then, can it repress the cancer cells' metastasis potential? To elucidate this issue, the present study was performed. LMP1-negative cell line HNE1 and LMP1-positive cell line HNE1-LMP1 were used as in vitro model. Cells (1 x 10(5)/mL) were cultured with or without 3 microM As2O3 for 48 h. Then the survival cells were collected to investigate their potential of colony formation, attachment, invasion, and migration. Both confocal immunofluorescence staining and Western blot were used to detect the changes of LMP1 expression. The changes of MMP-9 were examined by RT-PCR assay and Western blot. The results were as follow: i) the colony formation inhibition rate (75.41 +/- 3.9% in HNE1-LMP1 cells vs 37.89 +/- 4.9% in HNE1 cells), the rate of attachment (HNE1-LMP1 vs HNE1: 56.40 +/- 3.5 vs 65.87 +/- 5.9%), the invasion inhibitory rate (HNE1-LMP1 vs HNE1: 56.50 +/- 3.7 and 27.91 +/- 2.1%), and the migration inhibitory rate (HNE1-LMP1 vs HNE1: 48.70 +/- 3.9 vs 29.19 +/- 6.27%) were all significantly different between the two cell lines (P < 0.01). ii) LMP1 was down-regulated in As2O3-treated HNE1-LMP1 cells. iii) The reduction of MMP-9 was found in As2O3-treated groups, more evident in HNE1-LMP1 cells. Thus, we conclude that As2O3 can reduce metastasis potential of NPC cells, involving inhibition of MMP-9 expression. LMP1 were also reduced in this process and seemed to enhance anti-metastasis activity of As2O3.
C W Du; B G Wen; D R Li; X Peng; C Q Hong; J Y Chen; Z Z Lin; X Hong; Y C Lin; L X Xie; M Y Wu; H Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-04-20
Journal Detail:
Title:  Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]     Volume:  39     ISSN:  0100-879X     ISO Abbreviation:  Braz. J. Med. Biol. Res.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-05-01     Completed Date:  2007-02-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8112917     Medline TA:  Braz J Med Biol Res     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  677-85     Citation Subset:  IM    
Laboratory of Cancer Research, Cancer Hospital, Shantou University Medical College, Guang Dong, PR, China.
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MeSH Terms
Antineoplastic Agents / pharmacology*
Arsenicals / pharmacology*
Blotting, Western
Cell Line, Tumor
Cell Proliferation / drug effects
Cell Survival / drug effects
Fluorescent Antibody Technique
Gene Expression Regulation, Neoplastic
Matrix Metalloproteinase 9 / drug effects*,  genetics
Microscopy, Confocal
Nasopharyngeal Neoplasms / drug therapy*,  pathology
Neoplasm Invasiveness / pathology
Neoplasm Metastasis / pathology
Oxides / pharmacology*
RNA, Messenger / drug effects
Reverse Transcriptase Polymerase Chain Reaction
Viral Matrix Proteins / drug effects*,  genetics
Reg. No./Substance:
0/Antineoplastic Agents; 0/Arsenicals; 0/EBV-associated membrane antigen, Epstein-Barr virus; 0/Oxides; 0/RNA, Messenger; 0/Viral Matrix Proteins; 1327-53-3/arsenic trioxide; EC Metalloproteinase 9

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