Document Detail

Arsenic trioxide-induced hela cell death is partially prevented by K+ channel blockers.
MedLine Citation:
PMID:  16196273     Owner:  NLM     Status:  MEDLINE    
AIM: To investigate the effects of K+ channel blockers on arsenic trioxide-induced HeLa cell death. METHODS: Viability of HeLa cells was assessed by mitochondrial dehydrogenase activity using colorimetric MTT assay and the voltage-dependent K+ currents were recorded by using patch-clamp technique. RESULTS: Exposure of As2O3 (5 micromol x L(-1)) for 24 h caused marked HeLa cell death. The rest living cells after As2O3 24 h-incubation showed significant increase of K+ currents densities. At +80 mV, the densities of K+ currents (61 +/- 18) pA/10 pF (n = 8) in As2O3 24 h-incubation group were significantly more than that in the control group (38 +/- 10) pA/10 pF (n = 8, P < 0.05). The HeLa cells were prevented partially from As2O3-induced cell death by co-application for 24 h with typical voltage-dependent K+ channel blockers, 4-aminopyridine (3 mmol x L(-1)) or tetraethylammonium (5 mmol x L(-1)). 4-Aminopyridine (3 mmol x L(-1)) or tetraethylammonium (5 mmol x L(-1)) did not show any toxic effects on HeLa cells. CONCLUSION: Chronic treatment with As2O3 increased voltage-dependent K+ currents in HeLa cells and the cell death induced by As2O3 was reduced partially by voltage-dependent K+ channel blockers, 4-aminopyridine or tetraethylammonium.
De-Li Dong; Wei-Hua Song; Pei-Lin; Bao-Feng Yang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Yao xue xue bao = Acta pharmaceutica Sinica     Volume:  40     ISSN:  0513-4870     ISO Abbreviation:  Yao Xue Xue Bao     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-10-03     Completed Date:  2007-03-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  21710340R     Medline TA:  Yao Xue Xue Bao     Country:  China    
Other Details:
Languages:  eng     Pagination:  644-8     Citation Subset:  IM    
Department of Pharmacology, Bio-pharmaceutical Key Laboratory of Heilongjiang Province, Harbin Medical University, Harbin 150086, China.
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MeSH Terms
4-Aminopyridine / pharmacology*
Arsenicals / antagonists & inhibitors*,  pharmacology
Cell Death / drug effects
Hela Cells
Oxides / antagonists & inhibitors*,  pharmacology
Potassium Channel Blockers / pharmacology*
Potassium Channels, Voltage-Gated / drug effects
Tetraethylammonium / pharmacology*
Reg. No./Substance:
0/Arsenicals; 0/Oxides; 0/Potassium Channel Blockers; 0/Potassium Channels, Voltage-Gated; 1327-53-3/arsenic trioxide; 504-24-5/4-Aminopyridine; 66-40-0/Tetraethylammonium

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