| Arsenic species that cause release of iron from ferritin and generation of activated oxygen. | |
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MedLine Citation:
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PMID: 11068869 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The in vitro effects of four different species of arsenic (arsenate, arsenite, monomethylarsonic acid, and dimethylarsinic acid) in mobilizing iron from horse spleen ferritin under aerobic and anaerobic conditions were investigated. Dimethylarsinic acid (DMA(V)) and dimethylarsinous acid (DMA(III)) significantly released iron from horse spleen ferritin either with or without the presence of ascorbic acid, a strong synergistic agent. Ascorbic acid-mediated iron release was time-dependent as well as both DMA(III) and ferritin concentration-dependent. Iron release from ferritin by DMA(III)) alone or with ascorbic acid was not significantly inhibited by superoxide dismutase (150 or 300 units/ml). However, the iron release was greater under anaerobic conditions (nitrogen gas), which indicates direct chemical reduction of iron from ferritin by DMA(III), with or without ascorbic acid. Both DMA(V) and DMA(III)) released iron from both horse spleen and human liver ferritin. Further, the release of ferritin iron by DMA(III)) with ascorbic acid catalyzed bleomycin-dependent degradation of calf thymus DNA. These results indicate that exogenous methylated arsenic species and endogenous ascorbic acid can cause (a) the release of iron from ferritin, (b) the iron-dependent formation of reactive oxygen species, and (c) DNA damage. This reactive oxygen species pathway could be a mechanism of action of arsenic carcinogenesis in man. |
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Authors:
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S Ahmad; K T Kitchin; W R Cullen |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 382 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 2000 Oct |
Date Detail:
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Created Date: 2000-11-28 Completed Date: 2000-11-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 195-202 Citation Subset: IM |
Affiliation:
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Environmental Carcinogenesis Division, US Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arsenates / toxicity Arsenic / pharmacokinetics, toxicity* Arsenicals / adverse effects Arsenites / toxicity Cacodylic Acid / toxicity Carcinogens / toxicity Cattle Ferritins / chemistry, drug effects*, metabolism* Glutathione / metabolism Horses Humans Iron / metabolism* Kinetics Methylation Neoplasms / etiology Reactive Oxygen Species / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Arsenates; 0/Arsenicals; 0/Arsenites; 0/Carcinogens; 0/Reactive Oxygen Species; 124-58-3/monomethylarsonic acid; 15502-74-6/arsenite; 70-18-8/Glutathione; 7439-89-6/Iron; 7440-38-2/Arsenic; 75-60-5/Cacodylic Acid; 7778-39-4/arsenic acid; 9007-73-2/Ferritins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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