Document Detail

Ars2 links the nuclear cap-binding complex to RNA interference and cell proliferation.
MedLine Citation:
PMID:  19632182     Owner:  NLM     Status:  MEDLINE    
Here we identify a component of the nuclear RNA cap-binding complex (CBC), Ars2, that is important for miRNA biogenesis and critical for cell proliferation. Unlike other components of the CBC, Ars2 expression is linked to the proliferative state of the cell. Deletion of Ars2 is developmentally lethal, and deletion in adult mice led to bone marrow failure whereas parenchymal organs composed of nonproliferating cells were unaffected. Depletion of Ars2 or CBP80 from proliferating cells impaired miRNA-mediated repression and led to alterations in primary miRNA processing in the nucleus. Ars2 depletion also reduced the levels of several miRNAs, including miR-21, let-7, and miR-155, that are implicated in cellular transformation. These findings provide evidence for a role for Ars2 in RNA interference regulation during cell proliferation.
Joshua J Gruber; D Steven Zatechka; Leah R Sabin; Jeongsik Yong; Julian J Lum; Mei Kong; Wei-Xing Zong; Zhenxi Zhang; Chi-Kong Lau; Jason Rawlings; Sara Cherry; James N Ihle; Gideon Dreyfuss; Craig B Thompson
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell     Volume:  138     ISSN:  1097-4172     ISO Abbreviation:  Cell     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-27     Completed Date:  2009-08-17     Revised Date:  2014-09-12    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  328-39     Citation Subset:  IM    
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MeSH Terms
Arsenic / toxicity
Cell Line
Cell Proliferation*
Guanosine / analogs & derivatives,  metabolism
Nuclear Cap-Binding Protein Complex / metabolism*
Nuclear Proteins / metabolism*
RNA Interference*
Grant Support
K01 CA098092/CA/NCI NIH HHS; K01 CA098092-01A2/CA/NCI NIH HHS; K01 CA098092-02/CA/NCI NIH HHS; K01 CA098092-03/CA/NCI NIH HHS; K01 CA098092-04/CA/NCI NIH HHS; K01 CA098092-05/CA/NCI NIH HHS; K01 CA098092-06/CA/NCI NIH HHS; R01 CA092660/CA/NCI NIH HHS; R01 CA092660-09/CA/NCI NIH HHS; R01 CA099179/CA/NCI NIH HHS; R01 CA099179-07/CA/NCI NIH HHS; T32 AI007324/AI/NIAID NIH HHS; //Howard Hughes Medical Institute
Reg. No./Substance:
0/MicroRNAs; 0/Nuclear Cap-Binding Protein Complex; 0/Nuclear Proteins; 12133JR80S/Guanosine; 2140-77-4/7-methylguanosine; N712M78A8G/Arsenic
Comment In:
Cell. 2009 Jul 23;138(2):224-6   [PMID:  19632172 ]

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