Document Detail

Arrest of cell cycle progression of HeLa cells in the early G1 phase in K(+)-depleted conditions and its recovery upon addition of insulin and LDL.
MedLine Citation:
PMID:  8227179     Owner:  NLM     Status:  MEDLINE    
Cell cycle progression of synchronized HeLa cells was studied by measuring labeling of the nuclei with [3H]thymidine. The progression was arrested in a chemically defined medium in which K+ was replaced by Rb+ (Rb-CDM) but was restored upon addition of insulin and/or low density lipoprotein (LDL). Cells started DNA synthesis 12 hr after addition of insulin and/or LDL, regardless of the time of arrest, suggesting their arrest early in the G1 phase. After incubation of cells in Rb-CDM containing insulin or LDL singly for 3, 6, or 9 hr, replacement of the medium by that without an addition resulted in marked delay in entry of cells into the S phase, but in its replacement by medium containing both agents, the delay was insignificant. Synthesis of bulk protein, estimated as increase in the cell volume, was not strongly inhibited. From these results we conclude that cell cycle progression of HeLa cells in K(+)-depleted CDM is arrested early in the G1 phase and that the arrest is due to lack of some protein(s) required for entry into the S phase that is synthesized in the early G1 phase.
H Yamaguchi; K Hosokawa; Z L Jiang; A Takahashi; T Ikehara; H Miyamoto
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  53     ISSN:  0730-2312     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1993-12-09     Completed Date:  1993-12-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  13-20     Citation Subset:  IM    
Department of Physiology, School of Medicine, University of Tokushima, Japan.
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MeSH Terms
Cell Cycle* / drug effects
DNA / biosynthesis
G1 Phase*
Hela Cells
Insulin / pharmacology*
Lipoproteins, LDL / pharmacology*
Potassium / administration & dosage,  physiology*
Reg. No./Substance:
0/Lipoproteins, LDL; 11061-68-0/Insulin; 7440-09-7/Potassium; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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