Document Detail


Arrest of B lymphocyte terminal differentiation by CD40 signaling: mechanism for lack of antibody-secreting cells in germinal centers.
MedLine Citation:
PMID:  9655487     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Despite extensive research, the role of CD40 signaling in B cell terminal differentiation remains controversial. Here we show that CD40 engagement arrests B cell differentiation prior to plasma cell formation. This arrest is manifested at a molecular level as a reduction in mRNA levels of secretory immunoglobulin gene products such as mu(s) and J chain as well as the loss of the transcriptional regulator BLIMP-1. Furthermore, the inhibition of B cell differentiation by CD40 engagement could not be overcome by either mitogens or cytokines, but could be reversed by antibodies that interfere with the CD40/gp39 interaction. These data suggest that secretory immunoglobulin is not produced by B cells that are actively engaged by gp39-expressing T cells.
Authors:
T D Randall; A W Heath; L Santos-Argumedo; M C Howard; I L Weissman; F E Lund
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunity     Volume:  8     ISSN:  1074-7613     ISO Abbreviation:  Immunity     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-07-23     Completed Date:  1998-07-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9432918     Medline TA:  Immunity     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  733-42     Citation Subset:  IM    
Affiliation:
Trudeau Institute, Saranac Lake, New York 12983, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / immunology
Antigens, CD40 / immunology*
B-Lymphocytes / cytology,  immunology*
CD40 Ligand
Cell Differentiation / immunology
Coculture Techniques
Germinal Center / cytology,  immunology*
Humans
Ligands
Membrane Glycoproteins / immunology
Mice
Mice, Inbred C57BL
Repressor Proteins*
Signal Transduction / immunology*
Transcription Factors / immunology
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antigens, CD40; 0/Ligands; 0/Membrane Glycoproteins; 0/Prdm1 protein, mouse; 0/Repressor Proteins; 0/Transcription Factors; 138415-26-6/PRDM1 protein, human; 147205-72-9/CD40 Ligand

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