| Array comparative genomic hybridization of peripheral blood granulocytes of patients with myelodysplastic syndrome detects karyotypic abnormalities. | |
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MedLine Citation:
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PMID: 20551276 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The diagnosis of myelodysplastic syndromes (MDSs) relies largely on morphologic and karyotypic abnormalities, present in about 50% of patients with MDS. Array-based genomic platforms have identified copy number alterations in 50% to 70% of bone marrow samples of patients with MDS with a normal karyotype, suggesting a diagnostic role for these platforms. We investigated whether blood granulocytes harbor the same copy number alterations as the marrow of affected patients. Of 11 patients, 4 had cytogenetic abnormalities shown by conventional karyotyping involving chromosomes 5, 8, 11, 20, and X, and these changes were seen in the granulocytes of all 4 patients by using array comparative genomic hybridization (aCGH). Cryptic alterations were identified at a significantly higher level in marrow CD34+ cells compared with granulocytes (P < .0001). These data suggest that aCGH analysis of circulating granulocytes may be useful in detecting gross karyotypic alterations in patients with MDS when marrow examination has failed or not been done. |
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Authors:
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Suzanne M Vercauteren; Sandy Sung; Daniel T Starczynowski; Wan L Lam; Helene Bruyere; Douglas E Horsman; Peter Tsang; Heather Leitch; Aly Karsan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of clinical pathology Volume: 134 ISSN: 1943-7722 ISO Abbreviation: Am. J. Clin. Pathol. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-16 Completed Date: 2010-07-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370470 Medline TA: Am J Clin Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 119-26 Citation Subset: AIM; IM |
Affiliation:
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Department of Pathology, Vancouver General Hospital, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Aged, 80 and over Bone Marrow Cells / pathology Chromosome Aberrations* Comparative Genomic Hybridization / methods Gene Expression* Gene Expression Profiling Genetic Variation Genomics / methods Granulocytes / chemistry, pathology* Humans Middle Aged Myelodysplastic Syndromes / blood, genetics*, pathology* Oligonucleotide Array Sequence Analysis / methods Polymerase Chain Reaction |
| Grant Support | |
ID/Acronym/Agency:
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MOP 89976//Canadian Institutes of Health Research |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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