Document Detail


Array comparative genomic hybridization of peripheral blood granulocytes of patients with myelodysplastic syndrome detects karyotypic abnormalities.
MedLine Citation:
PMID:  20551276     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The diagnosis of myelodysplastic syndromes (MDSs) relies largely on morphologic and karyotypic abnormalities, present in about 50% of patients with MDS. Array-based genomic platforms have identified copy number alterations in 50% to 70% of bone marrow samples of patients with MDS with a normal karyotype, suggesting a diagnostic role for these platforms. We investigated whether blood granulocytes harbor the same copy number alterations as the marrow of affected patients. Of 11 patients, 4 had cytogenetic abnormalities shown by conventional karyotyping involving chromosomes 5, 8, 11, 20, and X, and these changes were seen in the granulocytes of all 4 patients by using array comparative genomic hybridization (aCGH). Cryptic alterations were identified at a significantly higher level in marrow CD34+ cells compared with granulocytes (P < .0001). These data suggest that aCGH analysis of circulating granulocytes may be useful in detecting gross karyotypic alterations in patients with MDS when marrow examination has failed or not been done.
Authors:
Suzanne M Vercauteren; Sandy Sung; Daniel T Starczynowski; Wan L Lam; Helene Bruyere; Douglas E Horsman; Peter Tsang; Heather Leitch; Aly Karsan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of clinical pathology     Volume:  134     ISSN:  1943-7722     ISO Abbreviation:  Am. J. Clin. Pathol.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-16     Completed Date:  2010-07-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370470     Medline TA:  Am J Clin Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  119-26     Citation Subset:  AIM; IM    
Affiliation:
Department of Pathology, Vancouver General Hospital, Canada.
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Bone Marrow Cells / pathology
Chromosome Aberrations*
Comparative Genomic Hybridization / methods
Gene Expression*
Gene Expression Profiling
Genetic Variation
Genomics / methods
Granulocytes / chemistry,  pathology*
Humans
Middle Aged
Myelodysplastic Syndromes / blood,  genetics*,  pathology*
Oligonucleotide Array Sequence Analysis / methods
Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
MOP 89976//Canadian Institutes of Health Research

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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