Document Detail


Array-based functional screening of heparin glycans.
MedLine Citation:
PMID:  22633407     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Array methodologies have become powerful tools for interrogation of glycan-protein interactions but have critically lacked the ability to generate cell response data. Here, we report the development of a slide-based array method exemplified by measurement of activation of fibroblast growth factor signaling by heparin saccharides. Heparan sulfate-deficient Swiss 3T3 cells were overlaid onto an aminosilane-coated slide surface onto which heparin saccharides had been spotted and immobilized. The cells were transiently stimulated with FGF2 and immunofluorescence measured to assess downstream ERK1/2 phosphorylation. Activation of this signaling pathway response was restricted to cells exposed to heparin saccharides competent to activate FGF2 signaling. Differential activation of the overlaid cells by different-sized heparin saccharides was demonstrated by quantitative measurement of fluorescence intensity. This "glycobioarray" platform has significant potential as a generic tool for functional glycomics screening.
Authors:
Tania M Puvirajesinghe; Yassir A Ahmed; Andrew K Powell; David G Fernig; Scott E Guimond; Jeremy E Turnbull
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chemistry & biology     Volume:  19     ISSN:  1879-1301     ISO Abbreviation:  Chem. Biol.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-28     Completed Date:  2012-09-24     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  9500160     Medline TA:  Chem Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  553-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Fibroblast Growth Factor 2 / metabolism
Fibroblast Growth Factors / metabolism*
Heparin / metabolism*
Mice
Microarray Analysis / methods*
Mitogen-Activated Protein Kinase 1 / metabolism
Mitogen-Activated Protein Kinase 3 / metabolism
Phosphorylation
Signal Transduction
Grant Support
ID/Acronym/Agency:
G117/423//Medical Research Council; //Biotechnology and Biological Sciences Research Council; //Medical Research Council
Chemical
Reg. No./Substance:
103107-01-3/Fibroblast Growth Factor 2; 62031-54-3/Fibroblast Growth Factors; 9005-49-6/Heparin; EC 2.7.11.24/Mitogen-Activated Protein Kinase 1; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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