| Array-based functional screening of heparin glycans. | |
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MedLine Citation:
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PMID: 22633407 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Array methodologies have become powerful tools for interrogation of glycan-protein interactions but have critically lacked the ability to generate cell response data. Here, we report the development of a slide-based array method exemplified by measurement of activation of fibroblast growth factor signaling by heparin saccharides. Heparan sulfate-deficient Swiss 3T3 cells were overlaid onto an aminosilane-coated slide surface onto which heparin saccharides had been spotted and immobilized. The cells were transiently stimulated with FGF2 and immunofluorescence measured to assess downstream ERK1/2 phosphorylation. Activation of this signaling pathway response was restricted to cells exposed to heparin saccharides competent to activate FGF2 signaling. Differential activation of the overlaid cells by different-sized heparin saccharides was demonstrated by quantitative measurement of fluorescence intensity. This "glycobioarray" platform has significant potential as a generic tool for functional glycomics screening. |
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Authors:
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Tania M Puvirajesinghe; Yassir A Ahmed; Andrew K Powell; David G Fernig; Scott E Guimond; Jeremy E Turnbull |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Chemistry & biology Volume: 19 ISSN: 1879-1301 ISO Abbreviation: Chem. Biol. Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-05-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9500160 Medline TA: Chem Biol Country: United States |
Other Details:
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Languages: eng Pagination: 553-8 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Elsevier Ltd. All rights reserved. |
Affiliation:
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Centre for Glycobiology, Department of Biochemistry and Cell Biology, Institute of Integrative Biology, The University of Liverpool, Liverpool L69 7ZB, UK. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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