Document Detail

Arp1, an actin-related protein, in Plasmodium berghei.
MedLine Citation:
PMID:  20580650     Owner:  NLM     Status:  MEDLINE    
Actin-related proteins (Arps) constitute a family of eukaryotic cytoskeletal proteins involved in such diverse events as cell motility, cytokinesis, vesicle transport, and chromatin remodelling. Previously, in a study of Plasmodium berghei gene expression in ookinetes and oocysts, we detected stage-specific increased expression of a gene encoding an Arp. Here we further characterize this gene and the encoded protein. We present a phylogenetic and three-dimensional modelling analysis as well as cell biological and genetic data that support classification of this gene as being an orthologue of the actin-related protein 1 (Arp1). This gene was found to be expressed in asexual stages as well as in the mosquito stages of the parasite, both on the transcript and protein level. Our attempts to delete the gene in the parasite for functional studies were unsuccessful, suggesting that it may be essential. The protein was localized apically of the nucleus in ookinetes, and in combination with the known function of Arp1 proteins, this suggests a role in vesicular transport. Expression of the gene in Saccharomyces cerevisiae resulted in toxic effects and interference with the yeast cytoskeleton.
Inga Siden-Kiamos; Herwig Schüler; Dimitris Liakopoulos; Christos Louis
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-16
Journal Detail:
Title:  Molecular and biochemical parasitology     Volume:  173     ISSN:  1872-9428     ISO Abbreviation:  Mol. Biochem. Parasitol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-07-19     Completed Date:  2010-10-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8006324     Medline TA:  Mol Biochem Parasitol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  88-96     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, N. Plastira 100, Vassilika Vouton, 700 13 Heraklion, Crete, Greece.
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MeSH Terms
Actins / genetics*,  metabolism
Amino Acid Sequence
Cell Nucleus / chemistry
Cluster Analysis
Cytoskeletal Proteins / genetics
Gene Deletion
Gene Expression Profiling
Genes, Essential
Genes, Protozoan
Mice, Inbred BALB C
Models, Molecular
Plasmodium berghei / genetics*,  physiology
Protein Structure, Tertiary
Protozoan Proteins / genetics*,  metabolism
Saccharomyces cerevisiae / genetics,  growth & development,  metabolism
Saccharomyces cerevisiae Proteins / genetics
Sequence Alignment
Sequence Homology, Amino Acid
Reg. No./Substance:
0/ARP1 protein, S cerevisiae; 0/Actins; 0/Cytoskeletal Proteins; 0/Protozoan Proteins; 0/Saccharomyces cerevisiae Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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