| Aromatase is increased in astrocytes in the presence of elevated pressure. | |
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MedLine Citation:
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PMID: 21047944 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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After traumatic brain injury (TBI), a progressive injury and death of neurons and glia leads to decreased brain function. Endogenous and exogenous estrogens may protect these vulnerable cells. In this study, we hypothesized that increased pressure leads to an increase in aromatase expression and estrogen production in astrocytes. In this study, we subjected rat glioma (C6) cells and primary cortical astrocytes to increased pressure (25 mm Hg) for 1, 3, 6, 12, 24, 48, and 72 h. Total aromatase protein and RNA levels were measured using Western analysis and RT-PCR, respectively. In addition, we measured aromatase activity by assaying estrone levels after administration of its precursor, androstenedione. We found that increased pressure applied to the C6 cells and primary cortical astrocytes resulted in a significant increase in both aromatase RNA and protein. To extend these findings, we also analyzed aromatase activity in the primary astrocytes during increased pressure. We found that increased pressure resulted in a significant (P < 0.01) increase in the conversion of androstenedione to estrone. In conclusion, we propose that after TBI, astrocytes sense increased pressure, leading to an increase in aromatase production and activity in the brain. These results may suggest mechanisms of brain estrogen production after increases in pressure as seen in TBI patients. |
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Authors:
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J W Gatson; J W Simpkins; K D Yi; A H Idris; J P Minei; J G Wigginton |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-11-03 |
Journal Detail:
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Title: Endocrinology Volume: 152 ISSN: 1945-7170 ISO Abbreviation: Endocrinology Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-24 Completed Date: 2011-01-31 Revised Date: 2013-02-27 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
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Languages: eng Pagination: 207-13 Citation Subset: AIM; IM |
Affiliation:
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Department of Surgery, University of Texas Southern Medical Center Dallas, Texas 75390-8579, USA. joshua.gatson@utsw.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Androstenedione
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metabolism Animals Aromatase / metabolism* Astrocytes / cytology, enzymology* Cell Line DNA Damage Estrone / metabolism Gene Expression Regulation, Enzymologic* Glioma Pressure* RNA / genetics, metabolism Rats |
| Grant Support | |
ID/Acronym/Agency:
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5 P50 GM21681-44/GM/NIGMS NIH HHS; 5 T32 GM08593-14/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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53-16-7/Estrone; 63-05-8/Androstenedione; 63231-63-0/RNA; EC 1.14.14.1/Aromatase |
| Comments/Corrections | |
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