Document Detail


Aromatase is increased in astrocytes in the presence of elevated pressure.
MedLine Citation:
PMID:  21047944     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
After traumatic brain injury (TBI), a progressive injury and death of neurons and glia leads to decreased brain function. Endogenous and exogenous estrogens may protect these vulnerable cells. In this study, we hypothesized that increased pressure leads to an increase in aromatase expression and estrogen production in astrocytes. In this study, we subjected rat glioma (C6) cells and primary cortical astrocytes to increased pressure (25 mm Hg) for 1, 3, 6, 12, 24, 48, and 72 h. Total aromatase protein and RNA levels were measured using Western analysis and RT-PCR, respectively. In addition, we measured aromatase activity by assaying estrone levels after administration of its precursor, androstenedione. We found that increased pressure applied to the C6 cells and primary cortical astrocytes resulted in a significant increase in both aromatase RNA and protein. To extend these findings, we also analyzed aromatase activity in the primary astrocytes during increased pressure. We found that increased pressure resulted in a significant (P < 0.01) increase in the conversion of androstenedione to estrone. In conclusion, we propose that after TBI, astrocytes sense increased pressure, leading to an increase in aromatase production and activity in the brain. These results may suggest mechanisms of brain estrogen production after increases in pressure as seen in TBI patients.
Authors:
J W Gatson; J W Simpkins; K D Yi; A H Idris; J P Minei; J G Wigginton
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-11-03
Journal Detail:
Title:  Endocrinology     Volume:  152     ISSN:  1945-7170     ISO Abbreviation:  Endocrinology     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-24     Completed Date:  2011-01-31     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  207-13     Citation Subset:  AIM; IM    
Affiliation:
Department of Surgery, University of Texas Southern Medical Center Dallas, Texas 75390-8579, USA. joshua.gatson@utsw.edu
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MeSH Terms
Descriptor/Qualifier:
Androstenedione / metabolism
Animals
Aromatase / metabolism*
Astrocytes / cytology,  enzymology*
Cell Line
DNA Damage
Estrone / metabolism
Gene Expression Regulation, Enzymologic*
Glioma
Pressure*
RNA / genetics,  metabolism
Rats
Grant Support
ID/Acronym/Agency:
5 P50 GM21681-44/GM/NIGMS NIH HHS; 5 T32 GM08593-14/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
53-16-7/Estrone; 63-05-8/Androstenedione; 63231-63-0/RNA; EC 1.14.14.1/Aromatase
Comments/Corrections

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