Document Detail


Aroma WaterLOGSY: a fast and sensitive screening tool for drug discovery.
MedLine Citation:
PMID:  21038321     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
One-dimensional NMR spectroscopy has proven to be a powerful technique for screening compound libraries in drug discovery. We report a novel water ligand-observed gradient spectroscopy (WaterLOGSY) pulse sequence, named Aroma WaterLOGSY, that selectively detects aromatic WaterLOGSY signals from compounds or ligands. In the Aroma WaterLOGSY, water magnetization is untouched after water excitation and utilizes the whole period of the remaining pulse sequence to relax back to the +z direction. Due to the phase cycling design, the water magnetization is allowed to relax for the period of two full scans before it gets inverted again. Therefore, the recycle delay can be significantly shortened. Within similar experimental time, Aroma WaterLOGSY shows approximately two times higher sensitivity than the standard scheme. This method also allows the use of non-deuterated reagents, thereby accelerating experimental set-up time for ligand-binding studies.
Authors:
Jun Hu; Per-Olof Eriksson; Gunther Kern
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Magnetic resonance in chemistry : MRC     Volume:  48     ISSN:  1097-458X     ISO Abbreviation:  Magn Reson Chem     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882600     Medline TA:  Magn Reson Chem     Country:  England    
Other Details:
Languages:  eng     Pagination:  909-11     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 John Wiley & Sons, Ltd.
Affiliation:
AstraZeneca R&D Boston, Waltham, MA 02451, USA. jun.hu2@astrazeneca.com
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