| Aroma WaterLOGSY: a fast and sensitive screening tool for drug discovery. | |
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MedLine Citation:
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PMID: 21038321 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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One-dimensional NMR spectroscopy has proven to be a powerful technique for screening compound libraries in drug discovery. We report a novel water ligand-observed gradient spectroscopy (WaterLOGSY) pulse sequence, named Aroma WaterLOGSY, that selectively detects aromatic WaterLOGSY signals from compounds or ligands. In the Aroma WaterLOGSY, water magnetization is untouched after water excitation and utilizes the whole period of the remaining pulse sequence to relax back to the +z direction. Due to the phase cycling design, the water magnetization is allowed to relax for the period of two full scans before it gets inverted again. Therefore, the recycle delay can be significantly shortened. Within similar experimental time, Aroma WaterLOGSY shows approximately two times higher sensitivity than the standard scheme. This method also allows the use of non-deuterated reagents, thereby accelerating experimental set-up time for ligand-binding studies. |
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Authors:
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Jun Hu; Per-Olof Eriksson; Gunther Kern |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Magnetic resonance in chemistry : MRC Volume: 48 ISSN: 1097-458X ISO Abbreviation: Magn Reson Chem Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9882600 Medline TA: Magn Reson Chem Country: England |
Other Details:
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Languages: eng Pagination: 909-11 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 John Wiley & Sons, Ltd. |
Affiliation:
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AstraZeneca R&D Boston, Waltham, MA 02451, USA. jun.hu2@astrazeneca.com |
Export Citation:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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