Document Detail

Argyrophilic nucleolar organizing regions and Ki67 equally reflect proliferation in fine needle aspirates of normal, hyperplastic, inflamed, and neoplastic canine lymph nodes (n = 101).
MedLine Citation:
PMID:  17939545     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The count of argyrophilic nucleolar organizing regions (AgNOR) has been considered a useful variable that reflects cellular proliferation in canine lymph nodes, but it has not been compared with other markers of proliferation.
HYPOTHESIS: Ki67 and AgNORs are equally useful as markers of tissue proliferation in fine needle aspirates of canine lymph nodes.
ANIMALS: A total of 101 dogs.
MATERIAL AND METHODS: Prospective, observational study of a convenience sample of dogs. Two smears were prepared for a May-Gruenwald-Giemsa stain and a Ki67/AgNOR double stain. In addition, CD3/CD79a immunostaining was performed when cytologic examination revealed a lymphoma. The dogs were grouped as normal (n = 26), reactive hyperplasia (n = 25), lymphadenitis (n = 31), and lymphoma (n = 19), based on the physical examination and the cytologic findings. The AgNOR count/cell, AgNOR area/cell and the percentage of cells staining positive for Ki67 were evaluated in 100-167 cells (median, 113 cells) by using automatic image analysis.
RESULTS: Mean (SD) AgNOR counts/cell were 1.36 +/- 0.19 in normal dogs, 1.55 +/- 0.26 in lymphadenitis, 1.65 +/- 0.32 in reactive hyperplasia, and 3.67 +/- 1.08 in lymphoma. The percentage of Ki67 positive cells was 2.67 +/- 0.99% in normal lymph nodes, 5.04 +/- 3.34% in lymphadenitis, 5.36 +/- 2.14% in reactive hyperplasia, and 30.2 +/- 10.8% in lymphoma. All variables were significantly higher in dogs with lymphoma compared with the other groups (P < .0001). The sensitivity and the specificity of the AgNOR count for diagnosing lymphoma were 95 and 96% at a cutoff value of >2.04 AgNORs/cell. The cutoff value for the Ki67 positive cells was >10.40% (sensitivity, 95%; specificity, 98%).
CONCLUSION AND CLINICAL IMPORTANCE: The results indicated that both AgNOR and Ki67 counts were good diagnostic tools for assessment of proliferation in aspirates of canine lymph nodes.
Natali B Bauer; Daniela Zervos; Andreas Moritz
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of veterinary internal medicine / American College of Veterinary Internal Medicine     Volume:  21     ISSN:  0891-6640     ISO Abbreviation:  J. Vet. Intern. Med.     Publication Date:    2007 Sep-Oct
Date Detail:
Created Date:  2007-10-17     Completed Date:  2007-12-06     Revised Date:  2014-07-30    
Medline Journal Info:
Nlm Unique ID:  8708660     Medline TA:  J Vet Intern Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  928-35     Citation Subset:  IM    
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MeSH Terms
Antigens, Nuclear / metabolism*
Area Under Curve
Biopsy, Fine-Needle / veterinary
Dog Diseases / diagnosis,  metabolism,  pathology*
Histocytochemistry / veterinary
Image Processing, Computer-Assisted
Ki-67 Antigen / metabolism
Lymphadenitis / diagnosis,  metabolism,  pathology,  veterinary*
Lymphoma / diagnosis,  metabolism,  pathology,  veterinary*
Nuclear Proteins / metabolism*
Predictive Value of Tests
Prospective Studies
ROC Curve
Sensitivity and Specificity
Reg. No./Substance:
0/Antigens, Nuclear; 0/Ki-67 Antigen; 0/Nuclear Proteins; 0/nucleolar organizer region associated proteins

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