Document Detail


Arginine and ornithine catabolism by Clostridium botulinum.
MedLine Citation:
PMID:  5335895     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Clostridium botulinum 62-A was shown to catabolize l-arginine via citrulline to ornithine, NH(3), and CO(2). The individual enzymes of the dihydrolase system were all demonstrated in extracts of cells, spores, and germinated spores. There was no liberation of urea from l-arginine, so no functional arginase enzyme is present, but there was some transamidinase activity in cell extracts. l-Ornithine was degraded at a significant rate by cells grown in an l-ornithine-supplemented medium; it was partially decarboxylated to putrescine and partially fermented to NH(3), CO(2), volatile acids, and delta-aminovaleric acid. Results from the fermentation of l-ornithine-C(14), -1-C(14), and -2-C(14) demonstrated that essentially all of the CO(2) was derived from carbon 1, and volatile acids from carbons 2 to 5. Assays for the products of l-ornithine-C(14) fermentation revealed that the volatile acids consisted of acetate, propionate, valerate, and butyrate (in order of decreasing concentrations), and that delta-amino-valerate was the primary reduced product. A small amount of citrulline was formed during the fermentation. The carbon and redox balances indicated that l-ornithine is fermented as a single substrate. Preliminary experiments demonstrated that the fermentation of l-ornithine is carried out by cell extracts with the production of volatile acids.
Authors:
B M Mitruka; R N Costilow
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  93     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1967 Jan 
Date Detail:
Created Date:  1967-05-10     Completed Date:  1967-05-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  295-301     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Arginase / metabolism
Arginine / metabolism*
Autoanalysis
Carbon Isotopes
Chromatography
Chromatography, Gas
Clostridium botulinum / enzymology,  metabolism*
Colorimetry
Fermentation
Ornithine / metabolism*
Transaminases / metabolism
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 7006-33-9/Ornithine; 74-79-3/Arginine; EC 2.6.1.-/Transaminases; EC 3.5.3.1/Arginase
Comments/Corrections

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