Document Detail

Arginine biosynthesis by Streptococcus bovis.
MedLine Citation:
PMID:  6051338     Owner:  NLM     Status:  MEDLINE    
The pathway of arginine biosynthesis in Streptococcus bovis was studied by radioactive tracer techniques. Cells were grown anaerobically with (14)CO(2) in a synthetic medium containing NH(4) (+) as the sole nitrogen source except for the trace present in nitrogen-containing vitamins. The protein fraction isolated from the labeled cells was acid-hydrolyzed, and (14)C-arginine was isolated from the protein hydrolysate by ion-exchange chromatography. The carboxyl carbon of the isolated arginine was removed with arginine decarboxylase, and the guanidino carbon was removed by simultaneous arginase-urease degradation. By manometric measurement and liquid scintillation counting of the CO(2) released by enzymatic degradation, 50% of the label was found in the carboxyl carbon and 50% in the guanidino carbon. Specific radioactivity determinations indicated that growth on (14)CO(2) resulted in twice as much label in arginine as with aspartate, glutamate, or lysine. These results are consistent with a glutamate --> ornithine --> citrulline pathway of arginine biosynthesis in S. bovis and provide further evidence for the synthesis of glutamate via the tricarboxylic acid cycle reactions from citrate through alpha-ketoglutarate.
R A Niederman; M J Wolin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  94     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1967 Oct 
Date Detail:
Created Date:  1967-12-15     Completed Date:  1967-12-15     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1002-6     Citation Subset:  IM    
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MeSH Terms
Ammonium Chloride / metabolism
Arginine / analysis,  biosynthesis*
Aspartic Acid / analysis
Carbon Dioxide / metabolism
Carbon Isotopes
Chromatography, Ion Exchange
Citric Acid Cycle
Glutamates / analysis
Lysine / analysis
Protein Hydrolysates / analysis
Streptococcus / metabolism*
Reg. No./Substance:
0/Carbon Isotopes; 0/Glutamates; 0/Protein Hydrolysates; 12125-02-9/Ammonium Chloride; 124-38-9/Carbon Dioxide; 56-84-8/Aspartic Acid; 56-87-1/Lysine; 74-79-3/Arginine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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