| Arginine biosynthesis by Streptococcus bovis. | |
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MedLine Citation:
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PMID: 6051338 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The pathway of arginine biosynthesis in Streptococcus bovis was studied by radioactive tracer techniques. Cells were grown anaerobically with (14)CO(2) in a synthetic medium containing NH(4) (+) as the sole nitrogen source except for the trace present in nitrogen-containing vitamins. The protein fraction isolated from the labeled cells was acid-hydrolyzed, and (14)C-arginine was isolated from the protein hydrolysate by ion-exchange chromatography. The carboxyl carbon of the isolated arginine was removed with arginine decarboxylase, and the guanidino carbon was removed by simultaneous arginase-urease degradation. By manometric measurement and liquid scintillation counting of the CO(2) released by enzymatic degradation, 50% of the label was found in the carboxyl carbon and 50% in the guanidino carbon. Specific radioactivity determinations indicated that growth on (14)CO(2) resulted in twice as much label in arginine as with aspartate, glutamate, or lysine. These results are consistent with a glutamate --> ornithine --> citrulline pathway of arginine biosynthesis in S. bovis and provide further evidence for the synthesis of glutamate via the tricarboxylic acid cycle reactions from citrate through alpha-ketoglutarate. |
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Authors:
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R A Niederman; M J Wolin |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bacteriology Volume: 94 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1967 Oct |
Date Detail:
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Created Date: 1967-12-15 Completed Date: 1967-12-15 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1002-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Ammonium Chloride
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metabolism Arginine / analysis, biosynthesis* Aspartic Acid / analysis Carbon Dioxide / metabolism Carbon Isotopes Chromatography, Ion Exchange Citric Acid Cycle Glutamates / analysis Lysine / analysis Protein Hydrolysates / analysis Streptococcus / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 0/Glutamates; 0/Protein Hydrolysates; 12125-02-9/Ammonium Chloride; 124-38-9/Carbon Dioxide; 56-84-8/Aspartic Acid; 56-87-1/Lysine; 74-79-3/Arginine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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