| Arginine availability modulates arginine metabolism and TNFα production in peritoneal macrophages from Zucker Diabetic Fatty rats. | |
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MedLine Citation:
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PMID: 22182949 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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BACKGROUND & AIMS: Excess weight and type 2 diabetes lead to increased susceptibility to infections. Our aim was to investigate the role of diabetes-induced decreased arginine (Arg) availability and of a possible dysregulation of Arg metabolism in macrophages favoring inflammation and dysimmunity via altered nitric oxide (NO) and cytokine productions. METHODS: Isolated peritoneal macrophages from Zucker Diabetic Fatty (ZDF) or lean rats were incubated with increasing Arg concentration (0-2 mM) and Arg metabolism and regulatory properties were studied. RESULTS: Inducible NO synthase (iNOS) expression did not vary with Arg concentration while NO production reached a maximum at 0.5 mM Arg, being significantly lower in macrophages from ZDF rats. Arginase I and II protein levels reached a maximum between 0.25 and 0.5 mM Arg in controls; in macrophages from ZDF rats arginase I was significantly lower and progressively increased up to 2 mM Arg while arginase II was not affected by Arg concentration. In parallel, Arg downregulated TNFα production in both groups and IL-6 only in control. CONCLUSIONS: This in vitro study shows that Arg metabolism is impaired in macrophages from diabetic-obese rats and that improving Arg availability for these cells restores NO production and contributes to the regulation of the inflammatory process. |
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Authors:
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Charlotte Breuillard; Linda Belabed; Sandra Bonhomme; Marie-Céline Blanc-Quintin; Nathalie Neveux; Rémy Couderc; Jean-Pascal De Bandt; Luc Cynober; Sylviane Darquy |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-17 |
Journal Detail:
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Title: Clinical nutrition (Edinburgh, Scotland) Volume: - ISSN: 1532-1983 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8309603 Medline TA: Clin Nutr Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved. |
Affiliation:
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Laboratory of Nutrition Biology, EA 4466, Paris Descartes University, France. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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