Document Detail


Arginase inhibition increases nitric oxide production in bovine pulmonary arterial endothelial cells.
MedLine Citation:
PMID:  14977627     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nitric oxide (NO) is produced by NO synthase (NOS) from L-arginine (L-Arg). Alternatively, L-Arg can be metabolized by arginase to produce L-ornithine and urea. Arginase (AR) exists in two isoforms, ARI and ARII. We hypothesized that inhibiting AR with L-valine (L-Val) would increase NO production in bovine pulmonary arterial endothelial cells (bPAEC). bPAEC were grown to confluence in either regular medium (EGM; control) or EGM with lipopolysaccharide and tumor necrosis factor-alpha (L/T) added. Treatment of bPAEC with L/T resulted in greater ARI protein expression and ARII mRNA expression than in control bPAEC. Addition of L-Val to the medium led to a concentration-dependent decrease in urea production and a concentration-dependent increase in NO production in both control and L/T-treated bPAEC. In a second set of experiments, control and L/T bPAEC were grown in EGM, EGM with 30 mM L-Val, EGM with 10 mM L-Arg, or EGM with both 10 mM L-Arg and 30 mM L-Val. In both control and L/T bPAEC, treatment with L-Val decreased urea production and increased NO production. Treatment with L-Arg increased both urea and NO production. The addition of the combination L-Arg and L-Val decreased urea production compared with the addition of L-Arg alone and increased NO production compared with L-Val alone. These data suggest that competition for intracellular L-Arg by AR may be involved in the regulation of NOS activity in control bPAEC and in response to L/T treatment.
Authors:
Louis G Chicoine; Michael L Paffett; Tamara L Young; Leif D Nelin
Related Documents :
8810597 - Nonadrenergic noncholinergic relaxation of human pulmonary arteries is partially mediat...
7516007 - Localization of endothelin immunoreactivity and demonstration of constrictory endotheli...
4084977 - Anti-spasmogenic effects of bencianol (zy15051) on human cerebral arteries in vitro.
2542057 - The endothelium modulates adrenergic neurotransmission to canine pulmonary arteries and...
8461627 - Aneurysms of coronary arteries in a patient with adult polycystic kidney disease: arter...
20593347 - Off-label use of stretchable polytetrafluoroethylene: overexpansion of synthetic shunts.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2004-02-20
Journal Detail:
Title:  American journal of physiology. Lung cellular and molecular physiology     Volume:  287     ISSN:  1040-0605     ISO Abbreviation:  Am. J. Physiol. Lung Cell Mol. Physiol.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-06-09     Completed Date:  2004-07-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100901229     Medline TA:  Am J Physiol Lung Cell Mol Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  L60-8     Citation Subset:  IM    
Affiliation:
Vascular Physiology Group and Department of Pediatrics, University of New Mexico Health Sciences Center, Albuquerque 87131, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Arginase / antagonists & inhibitors*,  genetics,  metabolism
Arginine / pharmacokinetics,  pharmacology
Cattle
Cells, Cultured
Dose-Response Relationship, Drug
Drug Combinations
Endothelium, Vascular / cytology,  metabolism*
Isoenzymes / genetics,  metabolism
Lipopolysaccharides / pharmacology
Nitrates / metabolism
Nitric Oxide / biosynthesis*
Nitrites / metabolism
Osmolar Concentration
Pulmonary Artery / cytology,  metabolism*
RNA, Messenger / metabolism
Time Factors
Tumor Necrosis Factor-alpha / pharmacology
Urea / antagonists & inhibitors
Valine / pharmacology*
Grant Support
ID/Acronym/Agency:
HL-04050/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Drug Combinations; 0/Isoenzymes; 0/Lipopolysaccharides; 0/Nitrates; 0/Nitrites; 0/RNA, Messenger; 0/Tumor Necrosis Factor-alpha; 10102-43-9/Nitric Oxide; 57-13-6/Urea; 7004-03-7/Valine; 74-79-3/Arginine; EC 3.5.3.1/Arginase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Influence of maturation on constrictive response to stimulation of C-fiber afferents in isolated gui...
Next Document:  Malnutrition impairs alveolar fluid clearance in rat lungs.