| Are telomeres a specific target for mutagenic attack by cytostatics in neoplastic cells? | |
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MedLine Citation:
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PMID: 15870889 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Damage to telomeres induced by cytostatic therapy theoretically could generate telomere shortening and, subsequently, induce an additional genomic instability in neoplastic cells. Model experiments were carried out to examine this hypothesis. Cells of the T-ALL derived cell line CCRF-CEM were exposed to various different concentrations of Bleomycin (BLM) or Mitomycin C (MMC) for various times. Telomere lengths of metaphase chromosomes of the exposed cells were compared with those without this exposure (controls). In addition, telomerase activity was determined with a TRAP assay under the given conditions using the BLM experiments as a model. Although slight changes of total telomere length could be found in single experiments, the differences between exposed and non-exposed cells were not significant. Also, a considerable telomerase activity was shown which, however, did not substantially differ between exposed and non-exposed cells. From these data it may be concluded that, at least in the examined cell line, telomeres are not a preferential target for this kind of mutagenic attack. |
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Authors:
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U Wick; E Gebhart |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of oncology Volume: 26 ISSN: 1019-6439 ISO Abbreviation: Int. J. Oncol. Publication Date: 2005 Jun |
Date Detail:
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Created Date: 2005-05-04 Completed Date: 2005-07-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9306042 Medline TA: Int J Oncol Country: Greece |
Other Details:
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Languages: eng Pagination: 1707-13 Citation Subset: IM |
Affiliation:
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Institute of Human Genetics, University of Erlangen-Nuremberg, Erlangen, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bleomycin
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toxicity Cell Cycle Cell Line, Tumor DNA Damage Humans Mitomycin / toxicity Mutagens / toxicity* Telomerase / antagonists & inhibitors, metabolism Telomere / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Mutagens; 11056-06-7/Bleomycin; 50-07-7/Mitomycin; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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