Document Detail

Are routine iron stains on bone marrow trephine biopsy specimens necessary?
MedLine Citation:
PMID:  15735158     Owner:  NLM     Status:  MEDLINE    
AIMS: To determine the role of Perls' staining in bone marrow trephine biopsy sections.
METHODS: The haemosiderin content of 155 Perls' stained, formic acid decalcified trephine biopsy sections was assessed and compared with Perls' stained aspirate samples in 105 cases and haematoxylin and eosin (H&E) stained biopsy sections in all cases.
RESULTS: An evaluable aspirate film with positive iron or at least seven negative particles was available for 105 biopsies. Only 71 of 95 cases with detectable aspirate iron had haemosiderin detectable on a Perls' stained section. None of 10 samples with a negative aspirate had a positive trephine biopsy. Haemosiderin was positive in 101 of the 155 Perls' stained sections, and was detectable on the H&E stained section in 71 of these cases. In five of 54 cases with negative Perls' staining, a small amount of haemosiderin was thought to be present on H&E staining.
CONCLUSIONS: Aspirate smears reflect bone marrow iron stores more reliably than formic acid decalcified trephine biopsy sections. The presence of iron in Perls' stained aspirates in 44% of cases with negative Perls' stained sections indicates that iron is often lost from sections during decalcification. However, 61% of cases with unassessable aspirate samples had a positive trephine biopsy Perls' stain, contributing useful clinical information about iron status. Preparation of Perls' stained sections only in cases in which aspirate samples are inadequate for iron assessment and no obvious haemosiderin is present in an H&E stained section could produce savings in staff time and reagent costs.
S E Stuart-Smith; D A Hughes; B J Bain
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical pathology     Volume:  58     ISSN:  0021-9746     ISO Abbreviation:  J. Clin. Pathol.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-28     Completed Date:  2005-03-29     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0376601     Medline TA:  J Clin Pathol     Country:  England    
Other Details:
Languages:  eng     Pagination:  269-72     Citation Subset:  AIM; IM    
Department of Haematology, St Mary's Hospital, Praed Street, London W2 1NY, UK.
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MeSH Terms
Bone Marrow / chemistry*,  pathology
Bone Marrow Examination / methods
Decalcification Technique
Hemosiderin / analysis*
Iron / analysis,  deficiency*
Staining and Labeling / methods
Unnecessary Procedures
Reg. No./Substance:
7439-89-6/Iron; 9011-92-1/Hemosiderin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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